A Conformational Study of the 10-23 DNAzyme via Programmed DNA Self-Assembly
A Conformational Study of the 10-23 DNAzyme via Programmed DNA Self-Assembly
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通过程序化 DNA 自组装对 10-23 DNAzyme 进行构象研究
DOI：
10.1039/d2cc01144a
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发表时间：
2022
影响因子：
4.9
通讯作者：
Mao, Chengde
中科院分区：
文献类型：
--
作者：
Mao, Dake;Li, Qian;Li, Qian;Wang, Pengfei;Mao, Chengde
通过原子力显微镜（AFM）测量了10-23 DNAzyme-substrate配合物的螺旋夹角。在此，我们设计了一种将DNAzyme - substrate复合物组装成周期性DNA 2D阵列的策略，该策略允许通过AFM成像和快速傅里叶变换（FFT）可靠地研究10-23 DNAzyme的构象。具体来说，催化核心的两个侧翼螺旋结构域之间的角度是通过二维阵列的重复距离来确定的。我们期望同样的策略可以普遍适用于其他核酸结构的研究。
This communication measures the inter-helical angle of the 10–23 DNAzyme–substrate complex by atomic force microscopy (AFM). Herein, we have devised a strategy to assemble the DNAzyme–substrate complex into a periodic DNA 2D array, which allows reliable study of the conformation of the 10–23 DNAzyme by AFM imaging and fast Fourier transform (FFT). Specifically, the angle between the two flanking helical domains of the catalytic core has been determined via the repeating distance of the 2D array. We expect that the same strategy can generally be applicable for studying other nucleic acid structures.