Regional gene therapy with a BMP-2-producing murine stromal cell line induces heterotopic and orthotopic bone formation in rodents
Regional gene therapy with a BMP-2-producing murine stromal cell line induces heterotopic and orthotopic bone formation in rodents
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DOI：
10.1002/jor.1100160309
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发表时间：
1998-05-01
影响因子：
2.8
通讯作者：
Stevenson, O
中科院分区：
文献类型：
--
作者：
Lieberman, JR;Le, LQ;Stevenson, O
将骨诱导蛋白连续递送到特定解剖部位的能力将促进骨折不愈合和与骨丢失相关的其他临床问题的治疗。我们已经开发了一种区域基因治疗的小鼠模型。用表达重组骨形态发生蛋白-2 cDNA的腺病毒感染的骨髓基质细胞系分泌生物活性骨形态发生蛋白-2。这些产生骨形态发生蛋白-2的细胞植入严重联合免疫缺陷小鼠的股四头肌后，能够诱导大量异位骨形成，并成功愈合裸鼠股骨大段缺损。这些研究表明，将骨诱导因子持续递送到特定解剖部位的区域基因治疗可以增强骨的形成和修复。
The ability to continuously deliver osteoinductive proteins to a specific anatomic site would facilitate the treatment of fracture nonunions and other clinical problems associated with bone loss. We have developed a murine model of regional gene therapy. A bone-marrow stromal cell line infected with an adenovirus expressing recombinant bone morphogenetic protein-2 cDNA secreted biologically active bone morphogenetic protein-2. These bone morphogenetic protein-2-producing cells were able to induce abundant heterotopic bone formation when implanted into the quadriceps muscle of severe combined immune deficient mice and also successfully healed large segmental femoral defects in nude rats. These studies demonstrate that regional gene therapy with continuous delivery of osteoinductive factors to a specific anatomic site can enhance the formation and repair of bone.