EXPERIMENTAL LYME-DISEASE IN DOGS PRODUCES ARTHRITIS AND PERSISTENT INFECTION
EXPERIMENTAL LYME-DISEASE IN DOGS PRODUCES ARTHRITIS AND PERSISTENT INFECTION
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DOI：
10.1093/infdis/167.3.651
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发表时间：
1993-03-01
影响因子：
6.4
通讯作者：
SUMMERS, BA
中科院分区：
文献类型：
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作者：
APPEL, MJG;ALLAN, S;SUMMERS, BA
通过暴露于伯氏疏螺旋体感染的蜱（硬蜱），在无特定病原体的比格犬中复制莱姆病。暴露于受感染的成年蜱后，血清转化率和疾病频率高于受感染的蜱。年轻的幼犬比老年犬更容易患上临床疾病。潜伏期2-5个月。急性复发性跛行伴纤维蛋白脓性关节炎是主要临床体征。犬恢复，但出现持续性轻度多关节炎。B。在恢复的犬中，伯氏菌持续存在至少一年。分离B。通过聚合酶链反应检测伯氏螺旋体，在蜱虫叮咬部位的皮肤活检中最为成功。抗B抗体。在暴露后4-6周，首先通过ELISA和蛋白质印迹检测到伯氏抗原。在17个月的观察期间，血清中的高浓度持续存在。与蜱虫感染相反，用培养的B. Burgdorferi导致血清转化，抗体持续时间较短，无临床疾病。
Lyme disease was reproduced in specific pathogen-free beagle dogs by exposure to Borrelia burgdorferi-infected ticks (Ixodes dammini). Seroconversion and disease frequency were higher after exposure to infected adult ticks than to infected nymphs. Young pups developed clinical disease more readily than older dogs. The incubation period lasted 2-5 months. Acute recurrent lameness with fibrinopurulent arthritis was the dominant clinical sign. Dogs recovered but developed persistent mild polyarthritis. B. burgdorferi persisted in recovered dogs for at least year. Isolation of B. burgdorferi and detection by polymerase chain reaction was most successful from skin biopsies at the site of the tick bite. Antibody to B. burgdorferi antigens was first detected by ELISA and Western blots by 4-6 weeks after exposure. High serum levels persisted during 17 months of observation. In contrast to infection from ticks, inoculation of dogs with cultured B. burgdorferi resulted in seroconversion with a shorter duration of antibody persistence and no clinical disease.