Inhibition of lymphocyte proliferation by antibodies to prolactin 1
Inhibition of lymphocyte proliferation by antibodies to prolactin 1
复制标题
催乳素 1 抗体抑制淋巴细胞增殖
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
E. Bernton
中科院分区：
文献类型：
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作者：
D. Hartmann;J. Holaday;E. Bernton
最近的体内研究表明，降低啮齿动物循环催乳素（PRL）的治疗会导致显著的免疫抑制。我们试图证明PRL对培养的淋巴细胞的相应直接刺激作用是不成功的。然而，抗垂体PRL抗体有效抑制小鼠和人淋巴细胞对T和B细胞有丝分裂原的增殖。使用IL 2和IL 4应答细胞系（CTLL-2和HT-2）进行的进一步研究表明，相同的抗PRL抗体可抑制对这些细胞因子生长因子的增殖反应。因此，PRL的抗体似乎阻断了这些细胞系的G1至GS相变中发生的事件，这些细胞系组成性表达生长因子受体。抗PRL抗体的抑制活性可通过加入纯化的人PRL或通过在亲和柱上固定化PRL来吸附。其他垂体激素抗体对CTLL-2细胞增殖无抑制作用。无血清培养基中淋巴细胞的增殖也受到抗PRL抗体的有效抑制，表明抗体效应不是由于PRL中和或培养血清补充剂中含有的其他因子所致。我们从这些数据中表明，与PRL具有同源性并被这些抗PRL抗体识别的蛋白质是由淋巴细胞产生的，并在其通过细胞周期的进展中起着关键作用。Hartmann，D. P的; Holcester，J. W.; Bernton，E. W.催乳素抗体对淋巴细胞增殖的抑制作用。FASEB J，3：2194 - 2202; 1989年。
Recent in vivo studies have shown that treatments that decrease circulating prolactin (PRL) in rodents result in significant immunosuppression. Our attempts to demonstrate corresponding direct stimulatory effects of PRL on cultured lymphocytes were unsuccessful. However, antibodies against pituitary PRL potently inhibited both murine and human lymphocyte proliferation in response to both T and B cell mitogens. Further studies using IL 2 and IL 4 responsive cell lines (CTLL‐2 and HT‐2) demonstrated that the same anti‐PRL antibodies inhibited the proliferative response to these cytokine growth factors. Thus, antibodies to PRL appear to block an event occurring in the G1 to GS phase transition of these cell lines, which constitutively express growth factor receptors. The inhibitory activity of anti‐PRL antibodies could be adsorbed by addition of purified human PRL or by immobilized PRL on an affinity column. Antibodies to other pituitary hormones were without inhibitory effect on CTLL‐2 cell proliferation. Proliferation of lymphocytes in serum‐free medium was also potently inhibited by anti‐PRL antibodies, suggesting that antibody effects were not due to neutralization of PRL or other factors contained in culture serum supplements. We suggest from these data that a protein with homology to PRL and recognized by these anti‐PRL antibodies is produced by lymphocytes and plays a critical role in their progression through the cell cycle.—Hartmann, D. P.; Holaday, J. W.; Bernton, E. W. Inhibition of lymphocyte proliferation by antibodies to prolactin. FASEB J, 3: 2194‐2202; 1989.
刀豆球蛋白 A 刺激的小鼠脾细胞产生具有催乳素样生物活性和免疫反应性的因子。
DOI：
10.1016/0006-291x(87)91020-5
发表时间：
1987
影响因子：
3.1
作者：
Montgomery,DW;Zukoski,CF;Shah,GN;Buckley,AR;Pacholczyk,T;Russell,DH
通讯作者：
Russell,DH
由克隆的 L3T4a II 类限制性 T 细胞产生的粒细胞巨噬细胞集落刺激因子诱导 HT-2 细胞增殖。
DOI：
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发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Woods,A;West,J;Rasmussen,R;Bottomly,K
通讯作者：
Bottomly,K
T 细胞无需血清即可生长。
DOI：
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发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Herzberg,VL;Smith,KA
通讯作者：
Smith,KA