The putative Na⁺/Cl⁻-dependent neurotransmitter/osmolyte transporter inebriated in the Drosophila hindgut is essential for the maintenance of systemic water homeostasis.
The putative Na⁺/Cl⁻-dependent neurotransmitter/osmolyte transporter inebriated in the Drosophila hindgut is essential for the maintenance of systemic water homeostasis.
复制标题
DOI：
10.1038/srep07993
复制
发表时间：
2015-01-23
影响因子：
4.6
通讯作者：
Li HS
中科院分区：
文献类型：
--
作者：
Luan Z;Quigley C;Li HS
Most organisms are able to maintain systemic water homeostasis over a wide range of external or dietary osmolarities. The excretory system, composed of the kidneys in mammals and the Malpighian tubules and hindgut in insects, can increase water conservation and absorption to maintain systemic water homeostasis, which enables organisms to tolerate external hypertonicity or desiccation. However, the mechanisms underlying the maintenance of systemic water homeostasis by the excretory system have not been fully characterized. In the present study, we found that the putative Na+/Cl−-dependent neurotransmitter/osmolyte transporter inebriated (ine) is expressed in the basolateral membrane of anterior hindgut epithelial cells. This was confirmed by comparison with a known basolateral localized protein, the α subunit of Na+-K+ ATPase (ATPα). Under external hypertonicity, loss of ine in the hindgut epithelium results in severe dehydration without damage to the hindgut epithelial cells, implicating a physiological failure of water conservation/absorption. We also found that hindgut expression of ine is required for water conservation under desiccating conditions. Importantly, specific expression of ine in the hindgut epithelium can completely restore disrupted systemic water homeostasis in ine mutants under both conditions. Therefore, ine in the Drosophila hindgut is essential for the maintenance of systemic water homeostasis.
登录
查看更多内容
影响因子：
3.8
作者：
Aikins, Michael J.;Schooley, David A.;Park, Yoonseong
通讯作者：
Park, Yoonseong
影响因子：
5.5
作者：
Blumenthal, EM
通讯作者：
Blumenthal, EM
影响因子：
2.7
作者：
AUDSLEY, N;PHILLIPS, JE
通讯作者：
PHILLIPS, JE
DOI：
10.1152/ajpregu.00208.2009
发表时间：
2010-03-01
影响因子：
2.8
作者：
Ianowski, Juan P.;Paluzzi, Jean-Paul;Orchard, Ian
通讯作者：
Orchard, Ian
影响因子：
5.2
作者：
Matzkin, Luciano M.;Watts, Thomas D.;Markow, Therese A.
通讯作者：
Markow, Therese A.