Quantitative DNA determinations by image analysis. I. Application to human pulmonary cytology.
Quantitative DNA determinations by image analysis. I. Application to human pulmonary cytology.
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通过图像分析定量 DNA 测定。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
R. Teplitz
中科院分区：
文献类型：
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作者：
H. Pak;V. Ashdjian;S. Yokota;R. Teplitz
本文介绍了图像分析在多种细胞脱氧核糖核酸(DNA)定量检测中的应用。来自Feulgen染色材料的视频信号的光密度被积分到整个细胞核区域，并获得与来自已知二倍体细胞的值相关的值。对三类材料进行分析：(1)已知单倍体、二倍体和四倍体核DNA的生物材料(标准)；(2)支气管上皮化生；(3)肺癌。图像分析的结果与生物标准的预期数据以及支气管系统化生和表皮样癌的显微分光光度数据相一致。讨论了该方法和其他定量方法在癌症筛查和生物学中的应用。
A description is made of the application of image analysis to quantitative deoxyribonucleic acid (DNA) determinations in a variety of cells. Optical density of the video signal from Feulgen-stained material was integrated across the area of the nucleus and values obtained related to values derived from known diploid cells. Analysis was performed on three classes of material: (1) biological material with known haploid, diploid and tetraploid nuclear DNA (standards), (2) metaplasias of the bronchial epithelium and (3) bronchogenic carcinomas. Results obtained with image analysis corresponded with expected data for the biologic standards and with microspectrophotometric data obtained for metaplasia and epidermoid carcinoma of the bronchial system. Application of this and other quantitative methods to cancer screening and biology is discussed.