Electrodeposition of anchored polypyrrole film on microelectrodes and stimulation of cultured cardiac myocytes
Electrodeposition of anchored polypyrrole film on microelectrodes and stimulation of cultured cardiac myocytes
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DOI：
10.1016/j.biomaterials.2006.11.034
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发表时间：
2007-03-01
期刊：
影响因子：
14
通讯作者：
Abe, Takashi
中科院分区：
文献类型：
--
作者：
Nishizawa, Matsuhiko;Nozaki, Hyuma;Abe, Takashi
将导电聚合物聚吡咯（PPy）电化学沉积在聚酰亚胺（PI）基底上的Pt微电极上。用十八烷基三氯硅烷自组装单分子层对PI表面进行预修饰，可以诱导PPy沿着P1表面的各向异性横向生长，提高PPy膜的粘附强度。PPy膜在电极周围的横向生长将整个膜锚定在衬底上。体外刺激培养的心肌细胞进行使用聚吡咯涂层微电极。微电极基底上的肌细胞被电缀合以形成薄片，并且在刺激时显示同步跳动。有效刺激0.8cm（2）心肌细胞层的阈值电荷约为0.2 μ C，大致相当于250 mV的膜去极化。(c)2006爱思唯尔有限公司版权所有。
The electrically conducting polymer polypyrrole (PPy) was electrochemically deposited onto Pt microelectrodes on a polyimide (PI) substrate. Pre-modification of the PI surface with a self-assembled monolayer of octadecyltrichlorosilane-induced anisotropic lateral growth of PPy along the P1 surface and enhanced adhesive strength of the PPy film. The lateral growth of PPy film around the electrode anchored the whole film to the substrate. External stimulation of cultured cardiac myocytes was carried out using the PPy-coated microelectrode. The myocytes on the microelectrode substrate were electrically conjugated to form a sheet, and showed synchronized beating upon stimulation. The threshold charge for effective stimulation of a 0.8cm(2) sheet of myocytes was around 0.2 mu C, roughly corresponding to a membrane depolarization of 250 mV. (c) 2006 Elsevier Ltd. All rights reserved.