The Bombyx mori sex pheromone biosynthetic pathway is not mediated by cAMP
The Bombyx mori sex pheromone biosynthetic pathway is not mediated by cAMP
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DOI：
10.1016/j.jinsphys.2007.02.013
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发表时间：
2007-08-01
影响因子：
2.2
通讯作者：
Matsumoto, Shogo
中科院分区：
文献类型：
--
作者：
Hull, J. Joe;Kajigaya, Ryosuke;Matsumoto, Shogo
在大多数蛾类中，性信息素的产生受信息素生物合成激活神经肽（PBAN）的调节。细胞外PBAN信号如何转化为生物反应一直是众多研究的焦点。在经典的信号转导方案中，激活的G蛋白将细胞外信号传递给下游效应分子，例如钙通道和腺苷酸环化酶。钙在PBAN信号传导中的作用已被清楚地证明，但cAMP的可能参与并不那么简单。虽然cAMP已被证明是必需的PBAN信号在大多数的向日葵物种，一直没有明确的证明其作用在家蚕。为了解决这个问题，我们利用简并RT-PCR技术从家蚕信息素腺体（PG）cDNA中克隆了两个Gs亚基，命名为P50Gs1和P50Gs2。这两个Gs蛋白在所有组织检查，并没有按照成人羽化上调。即使在PG匀浆中检测到与P50Gs1和P50Gs2的分子量近似对应的两条带，Gs拮抗剂NF 449对性信息素的产生没有影响。此外，PBAN刺激后未检测到细胞内cAMP水平的变化。(C)2007爱思唯尔有限公司版权所有。
In most moths, sex pheromone production is regulated by pheromone biosynthesis-activating neuropeptide (PBAN). How the extracellular PBAN signal is turned into a biological response has been the focus of numerous studies. In the classical scheme of signal transduction, activated G proteins relay the extracellular signal to downstream effector molecules such as calcium channels and adenylyl cyclase. The role of calcium in PBAN signaling has been clearly demonstrated, but the possible involvement of cAMP is not as straightforward. While cAMP has been shown to be necessary for PBAN signaling in most heliothine species, there has been no definitive demonstration of its role in Bombyx mori. To address this question, we used degenerate RT-PCR to clone two Gs subunits, designated P50Gs1 and P50Gs2, from R mori pheromone gland (PG) cDNAs. The two Gs proteins were expressed in all tissues examined and were not up-regulated in accordance with adult eclosion. Even though two bands corresponding to the approximate molecular weights of P50Gs1 and P50Gs2 were detected in PG homogenates, the Gs antagonist, NF449, had no effect on sex pheromone production. Furthermore, no changes in the intracellular cAMP levels were detected following PBAN stimulation. (C) 2007 Elsevier Ltd. All rights reserved.