Implantable arterial grafts from human fibroblasts and fibrin using a multi-graft pulsed flow-stretch bioreactor with noninvasive strength monitoring.
Implantable arterial grafts from human fibroblasts and fibrin using a multi-graft pulsed flow-stretch bioreactor with noninvasive strength monitoring.
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DOI：
10.1016/j.biomaterials.2010.09.019
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发表时间：
2011-01
期刊：
影响因子：
14
通讯作者：
Tranquillo, Robert T.
中科院分区：
文献类型：
--
作者：
Syedain, Zeeshan H.;Meier, Lee A.;Bjork, Jason W.;Lee, Ann;Tranquillo, Robert T.
组织工程化动脉的基础上截留的人真皮成纤维细胞在纤维蛋白凝胶产生完全生物血管移植物，具有天然动脉的圆周排列特性和必要的机械性能。开发了一种生物反应器，以在相同的培养基中调节6个移植物，同时经受类似的循环扩张和透壁流动，所述循环扩张和透壁流动由通过歧管分布在移植物管腔之间的脉冲流动产生。无创监测管腔压力和周向拉伸，并用于计算80-120 mmHg范围内的刚度，然后成功预测移植物爆破强度。在生物反应器培养期间逐渐缩短移植物的长度，以保持周向对齐并实现与自体动脉相当的机械各向异性。在7-9周的生物反应器培养后，纤维蛋白基移植物被成纤维细胞广泛地重塑为胶原蛋白和其他细胞外基质的周向排列的管，其破裂压力在1400-1600 mmHg的范围内，并且顺应性与天然动脉相当。组织缝线保持力也适用于大鼠模型中的植入，并且在绵羊模型中，聚乳酸缝合环截留在移植物的两端。在如此短的时间内用生物支架实现的强度对于工程动脉来说是前所未有的。
Tissue-engineered arteries based on entrapment of human dermal fibroblasts in fibrin gel yield completely biological vascular grafts that possess circumferential alignment characteristic of native arteries and essential to their mechanical properties. A bioreactor was developed to condition six grafts in the same culture medium while being subjected to similar cyclic distension and transmural flow resulting from pulsed flow distributed among the graft lumens via a manifold. The lumenal pressure and circumferential stretch were noninvasively monitored and used to calculate stiffness in the range of 80-120 mmHg and then to successfully predict graft burst strength. The length of the graft was incrementally shortened during bioreactor culture to maintain circumferential alignment and achieve mechanical anisotropy comparable to native arteries. After 7-9 weeks of bioreactor culture, the fibrin-based grafts were extensively remodeled by the fibroblasts into circumferentially-aligned tubes of collagen and other extracellular matrix with burst pressures in the range of 1400-1600 mmHg and compliance comparable to native arteries. The tissue suture retention force was also suitable for implantation in the rat model and, with poly(lactic acid) sewing rings entrapped at both ends of the graft, also in the ovine model. The strength achieved with a biological scaffold in such a short duration is unprecedented for an engineered artery.
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影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
DOI：
10.1115/1.2796072
发表时间：
1997-05-01
影响因子：
1.7
作者：
Barocas, VH;Tranquillo, RT
通讯作者：
Tranquillo, RT
影响因子：
37.8
作者：
Lloyd-Jones, Donald;Adams, Robert;Hong, Yuling
通讯作者：
Hong, Yuling
影响因子：
--
作者：
Solan, A;Mitchell, S;Niklason, L
通讯作者：
Niklason, L
影响因子：
3.4
作者：
Hoerstrup, SP;Zünd, G;Turina, MI
通讯作者：
Turina, MI