Incidence of infections dues to Escherichia coli strains producing extended spectrum betalactamase, in the Zou/Collines Hospital Centre (CHDZ/C) in Benin
Incidence of infections dues to Escherichia coli strains producing extended spectrum betalactamase, in the Zou/Collines Hospital Centre (CHDZ/C) in Benin
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DOI：
10.1016/j.medmal.2007.03.004
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发表时间：
2007-11-01
影响因子：
5
通讯作者：
Dramane, K.
中科院分区：
文献类型：
--
作者：
Ahoyo, A. T.;Baba-Moussa, L.;Dramane, K.
目标。-在6个月的时间里，从贝宁邹科林斯医院中心(CHDZ/C)的住院患者及其环境中收集了产超广谱β-内酰胺酶(ESBL)的大肠埃希菌(EC)菌株。这项研究的目的是确定超广谱β-内酰胺酶在贝宁一家二级医院(500张床位)的发病率，并描述其对抗生素的表型敏感性。方法：研究方法。-2005年5月15日至11月15日，收集了疑似医院感染患者的临床资料和样本。对分离的菌株进行鉴定，药敏试验，并用聚合酶链式反应分析ESBL基因blaTEM和blaSHV的存在。结果。共分离出肠杆菌197株，其中产超广谱β-内酰胺酶的肠杆菌占22%(32/143)。从环境中分离出46株大肠埃希菌，其中7株(15%)产超广谱β-内酰胺酶。除亚胺培南外，产超广谱β-内酰胺酶菌株对其他抗生素的耐药率均高于非产超广谱β-内酰胺酶菌株(P<；0.00001)，尤其对三代头孢菌素：头孢曲松、头孢他啶、头孢他啶的耐药率较高。从患者和环境中分离的ESBL菌株中均检出blaSHV和blaTEMESBL基因。结论。-这项研究表明，CHDZ/C医院不同病房的EC中存在ESBL基因，证明有必要实施严格的医院感染控制计划，并定期监测抗菌剂的耐药性。(C)2007年爱思唯尔·马森公司。数吨的浮油储量。
Objectives. - Over a 6-month period, extended-spectrum betalactamase (ESBL)-producing isolates of Escherichia coli (EC) were collected from in-patients and their environment at the Zou-Collines Hospital Centre (CHDZ/C) in Benin. The aim of this study was to determine the incidence of ESBL and to describe their phenotypic susceptibility to antibiotics in a secondary hospital (500 beds) in Benin. Methods. - From 15 May to 15 November 2005, clinical informations and samples were collected from patients suspected to have nosocomial infections. The isolates were identified, tested for antimicrobial susceptibility and analysed for the presence of ESBL genes blaTEM and blaSHV by PCR. Results. - One hundred ninety-seven enterobacteria were isolated from the clinical samples of 342 patients, these isolates included 143 EC and 32/143 (22%) of these isolates produced ESBL. Forty-six EC were isolated from the environment and 7 (15%) of them produced ESBL. Except for Imipenem for which the difference was not significant, the isolates producing ESBL were more resistant to the other antibiotics (especially to third generation cephalosporins: Ceftriaxone, Cefotaxime, Ceftazidime (P < 0.00001)) than non-ESBL producing isolates. Both ESBL genes blaSHV and blaTEM were identified in the EC ESBL strains from patient and from the environment. Conclusion. - This study shows the presence of ESBL genes among EC in various wards of the CHDZ/C hospital proving that there is a need to implement a strict hospital infection control program and a regular surveillance of resistance to antimicrobial agents. (c) 2007 Elsevier Masson SAS. Tons droits reserves.