Low-intensity focused ultrasound (LIFU)-induced acoustic droplet vaporization in phase-transition perfluoropentane nanodroplets modified by folate for ultrasound molecular imaging.
Low-intensity focused ultrasound (LIFU)-induced acoustic droplet vaporization in phase-transition perfluoropentane nanodroplets modified by folate for ultrasound molecular imaging.
复制标题
低强度聚焦超声 (LIFU) 诱导叶酸修饰的相变全氟戊烷纳米液滴中的声学液滴蒸发，用于超声分子成像。
DOI：
10.2147/ijn.s122667
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发表时间：
2017
影响因子：
8
通讯作者：
Du Z
中科院分区：
文献类型：
--
作者：
Liu J;Shang T;Wang F;Cao Y;Hao L;Ren J;Ran H;Wang Z;Li P;Du Z
常用的超声（US）分子探针，如靶向微泡和全氟化碳乳剂，存在许多固有的问题，包括US可视化和颗粒穿透之间的冲突。本研究描述了一种用于肿瘤分子成像的新型超声分子探针--相变叶酸靶向全氟戊烷纳米液滴（FA-ND）的成功制备。值得注意的是，这些FA-ND可以由低强度聚焦US（LIFU）超声处理触发，在体外B模式和对比度增强US模式下提供出色的US增强。1，1 ′-双十八烷基-3，3，3 ′，3 ′ -四甲基吲哚三羧酸碘标记的FA-NDs静脉注射SKOV 3荷瘤裸鼠后，可在肿瘤部位聚集。FA-NDs注射后LIFU超声处理在肿瘤区域表现出显著的US对比增强。总之，将我们精心开发的FA-ND与LIFU超声处理相结合，为叶酸受体过表达肿瘤的US分子成像提供了一种潜在的方案。
The commonly used ultrasound (US) molecular probes, such as targeted microbubbles and perfluorocarbon emulsions, present a number of inherent problems including the conflict between US visualization and particle penetration. This study describes the successful fabrication of phase changeable folate-targeted perfluoropentane nanodroplets (termed FA-NDs), a novel US molecular probe for tumor molecular imaging with US. Notably, these FA-NDs can be triggered by low-intensity focused US (LIFU) sonication, providing excellent US enhancement in B-mode and contrast-enhanced US mode in vitro. After intravenous administration into nude mice bearing SKOV3 ovarian carcinomas, 1,1′-dioctadecyl-3,3,3′,3′ -tetramethylindotricarbocya-nine iodide-labeled FA-NDs were found to accumulate in the tumor region. FA-NDs injection followed by LIFU sonication exhibited remarkable US contrast enhancement in the tumor region. In conclusion, combining our elaborately developed FA-NDs with LIFU sonication provides a potential protocol for US molecular imaging in folate receptor-overexpressing tumors.