Analysis of propionate-degrading consortia from agricultural biogas plants.
Analysis of propionate-degrading consortia from agricultural biogas plants.
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分析农业沼气植物的丙酸酯联盟。
DOI：
10.1002/mbo3.386
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发表时间：
2016-12
期刊：
影响因子：
3.4
通讯作者：
König H
中科院分区：
文献类型：
--
作者：
Ahlert S;Zimmermann R;Ebling J;König H
为了研究农业沼气厂丙酸盐降解菌群的组成，从不同来源的沼气厂中筛选出4个丙酸盐降解菌群（Ap 1a、N12、G12和Wp 2a）。将聚生体分批培养2-4年，然后在为期8周的分批实验中分析丙酸盐降解过程中的微生物演替。群落的变化表明，硫酸还原互养菌、隐藻属/ Pelotomaculum sp.，和“Cloacamonas sp.”在降低丙酸盐浓度的过程中。产甲烷菌主要为甲烷八叠球菌属、甲烷菌属和甲烷菌属。由于普遍存在互养型乙酸盐氧化物种Taceranaerobium acetatoxydans和潜在的自养型同型产乙酸细菌（Moorella sp.，产热菌属（Thermacetogenium sp.），指出了在稳定和有效的丙酸盐降解中理论上涉及互养乙酸盐氧化和自养同型乙酸盐生成。考虑到在不同氢分压下的理论吉布斯自由能值，值得注意的是，互养乙酸氧化和自养同型乙酸有可能抵消不利的氢分压波动，稳定最可能的连续和稳定的丙酸降解。
In order to investigate the propionate‐degrading community of agricultural biogas plants, four propionate‐degrading consortia (Ap1a, N12, G12, and Wp2a) were established from different biogas plants which were fed with renewable resources. The consortia were cultivated in a batch for a period of 2–4 years and then analyzed in an 8‐week batch experiment for microbial succession during propionate degradation. Community shifts showed considerable propagation of Syntrophobacter sulfatireducens, Cryptanaerobacter sp./Pelotomaculum sp., and “Candidatus Cloacamonas sp.” in the course of decreasing propionate concentration. Methanogenic species belonged mainly to the genera Methanosarcina, Methanosaeta, and Methanoculleus. Due to the prevalent presence of the syntrophic acetate‐oxidizing species Tepidanaerobacter acetatoxydans and potentially autotrophic homoacetogenic bacteria (Moorella sp., Thermacetogenium sp.), a theoretical involvement of syntrophic acetate oxidation and autotrophic homoacetogenesis in stable and efficient propionate degradation was indicated. Considering theoretical Gibbs free energy values at different hydrogen partial pressures, it is noticeable that syntrophic acetate oxidation and autotrophic homoacetogenesis have the potential to counterbalance adverse hydrogen partial pressure fluctuations, stabilizing most probably continuous and stable propionate degradation.