Human rhinovirus 87 identified as human enterovirus 68 by VP4-based molecular diagnosis
Human rhinovirus 87 identified as human enterovirus 68 by VP4-based molecular diagnosis
复制标题
DOI：
10.1159/000065866
复制
发表时间：
2002-05-01
期刊：
影响因子：
4.6
通讯作者：
Takeda, N
中科院分区：
文献类型：
--
作者：
Ishiko, H;Miura, R;Takeda, N
人类鼻病毒（hrv）是呼吸道感染的主要原因。我们建立了一种基于逆转录聚合酶链反应（RT-PCR）和基于vp4的系统发育分析的hrv诊断方法。一组用于人肠病毒（ev） RT-PCR的引物似乎能够扩增所有hrv原型株，每个原型株产生一个530-by的片段。唯一的例外是HRV87，它产生了一个650 bp的片段，在人类ev中观察到。HRV-87的VP4核苷酸序列与人EV-68的核苷酸同源性超过97%，与人EV-D的原型株形成单系聚类。在66例人类ev和12例hrv样本中，HRV-87与人类EV-D的另一成员EV-70的同源性次高（76.8%）。因此，应将HRV-87重新归类为包含人类EV-68的聚类。版权所有(C) 2002 S. Karger AG，巴塞尔。
Human rhinoviruses (HRVs) are the major cause of respiratory infections. We developed a diagnostic method for HRVs based on the reverse-transcription polymerase chain reaction (RT-PCR) and VP4-based phylogenetic analysis. A set of primers used in the RT-PCR of human enteroviruses (EVs) appeared to be capable of amplifying all prototype strains of HRVs, each of which generated a 530-by fragment. The single exception was HRV87, which generated a 650-bp fragment, as observed in human EVs. The VP4 nucleotide sequence of HRV-87 showed more than 97% nucleotide identity with human EV-68, and formed a monophyletic cluster along with the prototype strain of EV-68 in the human EV-D cluster. HRV-87 showed the second highest homology (76.8%) with EV-70, another member of the human EV-D, in a sample of 66 human EVs and 12 HRVs. Therefore, HRV-87 should be reclassified into the cluster containing human EV-68. Copyright (C) 2002 S. Karger AG, Basel.