Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood
Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood
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DOI：
10.1007/978-1-4939-2498-1_17
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Yu, Di
中科院分区：
文献类型：
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作者：
Wei, Yunbo;Feng, Jinhong;Yu, Di
滤泡辅助性T（Tfh）细胞是辅助性T细胞亚群，定位于次级淋巴器官内的生殖中心。它们支持B细胞产生高亲和力抗体并产生B细胞记忆。相比之下，滤泡调节T（Tfr）细胞定位于生发中心以抑制B细胞反应。Tfh和Tfr细胞协调调节抗体应答，在疫苗接种、传染病控制和自身免疫性疾病的发展中发挥关键作用。虽然真正的Tfh和Tfr细胞在人体血液中几乎检测不到，但血液中循环的Tfh和Tfr记忆细胞可用于研究它们在健康和疾病中的功能。我们已经开发了一种全面的流式细胞术分析，以确定不同的循环Tfh和Tfr群体内CXCR 5（+）CD 4（+）T细胞群体在人血液中，基于众多的细胞表面标志物，包括CD 25，CD 127，CCR 7，PD-1，CXCR 3，和CCR 6。
Follicular helper T (Tfh) cells are the helper T-cell subset that localizes in germinal centers within secondary lymphoid organs. They support B cells to produce high-affinity antibodies and generate B-cell memory. By contrast, follicular regulatory T (Tfr) cells localize in germinal centers to suppress B-cell responses. Coordinately regulating antibody responses, Tfh and Tfr cells play a critical role for vaccination, infectious disease control, and the development of autoimmune diseases. Although bona fide Tfh and Tfr cells are hardly detected in human blood, circulating Tfh and Tfr memory cells in blood can be used to investigate their functions in health and disease. We have developed a comprehensive flow cytometric analysis to define different circulating Tfh and Tfr populations within CXCR5(+) CD4(+) T-cell population in human blood, based on numerous cell surface markers including CD25, CD127, CCR7, PD-1, CXCR3, and CCR6.