Expression of the Homeobox‐containing Gene En‐2 During the Development of the Chick Central Nervous System
Expression of the Homeobox‐containing Gene En‐2 During the Development of the Chick Central Nervous System
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鸡中枢神经系统发育过程中同源盒基因En-2的表达
DOI：
10.1111/j.1460-9568.1995.tb00681.x
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发表时间：
1995
影响因子：
3.4
通讯作者：
R. Alvarado
中科院分区：
文献类型：
--
作者：
S. Millet;R. Alvarado
用单克隆抗体4D9分析了含同种异构体基因En - 2在胚发生过程中在鸡中枢神经系统中的表达。证实先前的研究，En - 2蛋白的早期表达[始于汉堡包和汉密尔顿（HH9）的第9期]仅限于包含小脑原基、峡区和中脑谷纹的神经管的一部分，并从位于中脑-后脑收缩周围的高点形成尾侧和喙侧的双梯度下降。在整个胚胎发生过程中，峡部小脑区包含所有En - 2阳性的萌发细胞和绝大多数En - 2阳性的有丝分裂后神经元。然而，随着有丝分裂后神经元的出现，En - 2表达也发生在该区域之外：在2 - 4菱形体（HH20和HH30之间）的两列非运动神经元细胞中，从HH24开始，整个腰椎和胸段脊髓的灰质中，除了腹侧运动神经元柱。本研究详细描述了在HH30-32[胚胎期(E) 71, HH37 （E11）和HH46 （E21，孵化期）]的mes -峡部-小脑区En - 2的表达。这使得具有En - 2异质表达模式的细胞组可视化成为可能，这在En - 2表达强度、标记细胞的分布和基因的时间调节方面对每个组都是特异性的。酪氨酸羟化酶抗血清表明，酪氨酸羟化酶和En - 2蛋白在桥脚核尾部、腹侧区和中央灰斑中共表达，而在蓝斑中不表达。在小脑中，当分子层中间神经元和颗粒细胞在胚胎发生末期开始表达该基因时，位于浦肯野细胞深核和副矢状带的第一个表达被下调。最后，在孵化时，En‐2的表达允许在小脑中观察到位于浦肯野细胞周围的小En‐2阴性细胞群，这些细胞可能对应于鸡/鹌鹑嵌合体中描述的起源不同于大部分颗粒神经元的起源。
The expression of the homeobox‐containing gene En‐2 was analysed with the monoclonal antibody 4D9 in the chick central nervous system throughout embryogenesis. Confirming previous studies, early expression of the En‐2 protein [beginning at stage 9 of Hamburger and Hamilton (HH9)] is restricted to a portion of the neural tube containing the primordia of the cerebellum, the isthmic region and the mesencephalic grisea, and forms a double gradient decreasing both caudally and rostrally from a high point located around the midbrain‐hindbrain constriction. This mes‐isthmo‐cerebellar region contains all the En‐2‐positive germinative cells and the great majority of the En‐2‐positive postmitotic neurons throughout embryogenesis. Nevertheless, as the postmitotic neurons appear, En‐2 expression also occurs outside this region: in two columns of non‐motoneuron cells in rhombomeres two to four (between HH20 and HH30) and, from HH24 onwards, throughout the grey matter of the lumbar and thoracic spinal cord, with the exception of the ventral motoneuron columns. Here, a detailed description of En‐2 expression is provided for the mes‐isthmo‐cerebellar region at stages HH30–32 [embryonic day (E) 71, HH37 (E11) and HH46 (E21, hatching). This allows the visualization of cellular groups with heterogeneous patterns of En‐2 expression, which are specific for each group in the intensity of En‐2 expression, the distribution of the labelled cells and the temporal regulation of the gene. The use of tyrosine hydroxylase antiserum shows coexpression of the tyrosine hydroxylase enzyme and En‐2 protein in the caudal part of the nuclei tegmenti pedunculo‐pontinus, the area ventralis of Tsai and the substantia grisea centralis, but not in the locus coeruleus. In the cerebellum, the first expression, which is located in the deep nuclei and parasagittal bands of Purkinje cells, is down‐regulated when the molecular layer interneurons and the granular cells begin to express the gene, at the end of embryogenesis. Finally, at hatching, En‐2 expression permits the visualization in the cerebellum of a population of small En‐2‐negative cells located around the Purkinje cells that may correspond to those described in chick/quail chimaeras as having an origin different from that of the bulk of granular neurons.
影响因子：
4.6
作者：
K. Millen;W. Wurst;K. Herrup;A. Joyner
通讯作者：
K. Millen;W. Wurst;K. Herrup;A. Joyner
影响因子：
56.9
作者：
JOYNER, AL;HERRUP, K;ROSSANT, J
通讯作者：
ROSSANT, J
基因表达模式的变化定义了小脑颗粒神经元分化的四个阶段。
DOI：
10.1242/dev.117.1.97
发表时间：
1993
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Kuhar,SG;Feng,L;Vidan,S;Ross,ME;Hatten,ME;Heintz,N
通讯作者：
Heintz,N