Small-molecule fluorescent probes for specific RNA targets
Small-molecule fluorescent probes for specific RNA targets
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DOI：
10.1039/c1cc10393h
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发表时间：
2011-01-01
影响因子：
4.9
通讯作者：
Uesugi, Motonari
中科院分区：
文献类型：
--
作者：
Murata, Asako;Sato, Shin-ichi;Uesugi, Motonari
开发了一种使用小分子作为荧光探针来检测特定mrna的方法。在这种方法中，荧光团-猝灭剂偶联物的荧光通过mRNA适体标签与分子猝灭剂片段的结合而恢复。该方法允许在体外实时检测mRNA转录物。
A method was developed that uses small molecules as fluorescent probes to detect specific mRNAs. In this approach, the fluorescence of fluorophore-quencher conjugates is restored by the binding of an mRNA aptamer tag to the quencher segment of the molecules. The method allows real-time detection of mRNA transcripts in vitro.