Dendritic cell uptake of iron-based magnetic nanoparticles
Dendritic cell uptake of iron-based magnetic nanoparticles
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DOI：
10.1016/j.cellbi.2008.04.001
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发表时间：
2008-08-01
影响因子：
3.9
通讯作者：
Tres, A.
中科院分区：
文献类型：
--
作者：
Goya, G. F.;Marcos-Campos, I.;Tres, A.
我们研究了磁性纳米颗粒(NPs)内化到树突状细胞(DC)中，以评估颗粒的最终位置和培养细胞的活力。这些颗粒由覆盖着碳的金属铁芯组成，对DC没有毒性作用，对它们的生存能力也没有影响。我们发现，经过24小时的培养，成熟的DC能够掺入大小从10 nm到约200 nm的磁性纳米颗粒。我们描述了一种分离装载有NPs的细胞的方法，并通过电子显微镜和磁性测量对所得材料进行分析。研究发现，NPs内化在溶酶体中，提供了一个很大的磁信号。我们的结果表明，用适当功能化的磁性纳米颗粒负载DC可能是一种在癌症诊断和治疗中改进矢量化的有前途的策略。(C)2008年国际细胞生物学联合会。爱思唯尔有限公司出版。保留所有权利。
We have investigated the internalization of magnetic nanoparticles (NPs) into dendritic cells (DCs) in order to assess both the final location of the particles and the viability of the cultured cells. The particles, consisting of a metallic iron core covered with carbon, showed no toxic effects on the DCs and had no effect in their viability. We found that mature DCs are able to incorporate magnetic nanoparticles in a range of size from 10 nm to ca. 200 nm, after 24 h of incubation. We describe a method to separate cells loaded with NPs, and analyze the resulting material by electron microscopy and magnetic measurements. It is found that NPs are internalized in lysosomes, providing a large magnetic signal. Our results suggest that loading DCs with properly functionalized magnetic NPs could be a promising strategy for improved vectorization in cancer diagnosis and treatment. (c) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.