Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1.
Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1.
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DOI：
10.1590/s1517-838220120001000019
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
Zhang D
中科院分区：
文献类型：
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作者：
Hao Z;Cai Y;Liao X;Zhang X;Fang Z;Zhang D
本研究报告了最近从土壤中分离出的一种新型细菌菌株对几丁质酶生产的统计中值优化，该菌株被命名为梅园几丁质分解菌 SYBC-H1。采用Plackett-Burman法和响应面法(RSM)组成的序贯统计方法来提高几丁质酶的发酵产量，其中首先使用菊粉作为有效碳源。结果，优化后的培养基获得了最大几丁质酶活性5.17 U/mL，是基础培养基的15.5倍。在优化的营养水平下进行了三次重复验证实验，表明其与预测值非常吻合。
The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.