Effect of interaction between 5-azacytidine and DNA (cytosine-5) methyltransferase on C-to-G and C-to-T mutations in Escherichia coli
Effect of interaction between 5-azacytidine and DNA (cytosine-5) methyltransferase on C-to-G and C-to-T mutations in Escherichia coli
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DOI：
10.1016/s0027-5107(99)00099-8
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发表时间：
1999-08-11
影响因子：
2.3
通讯作者：
Cupples, CG
中科院分区：
文献类型：
--
作者：
Doiron, KMJ;Lavigne-Nicolas, J;Cupples, CG
本研究的目的是确定Dcm胞嘧啶甲基转移酶对大肠杆菌5-氮杂胞苷（5-azaC）诱变的影响。在存在和不存在Dcm的情况下，我们使用Lac还原试验来测量lacZ基因中单个甲基化胞嘧啶的C-to-G和C-to-T突变。C-to-G突变由5-azaC刺激，但在很大程度上独立于Dcm。相比之下，无论是野生型细胞还是dcm细胞，5-azaC都不会刺激C-to-T突变。然而，在含有Dcm但在非常短的补丁修复中有缺陷的细胞中，通常高频率的自发C-to-T突变以剂量依赖的方式被类似物降低。(C) 1999 Elsevier Science B.V.版权所有
The purpose of this study was to determine the effect of the Dcm cytosine methyltransferase on 5-azacytidine (5-azaC) mutagenesis in Escherichia coli. We used a Lac reversion assay to measure C-to-G and C-to-T mutations at a single, methylatable cytosine in the lacZ gene, in the presence and absence of Dcm. C-to-G mutations are stimulated by 5-azaC but are largely independent of Dcm. In contrast, C-to-T mutations are not stimulated by 5-azaC in either wild type or dcm cells. However, in cells which contain Dcm but are defective in very short patch repair, the normally high frequency of spontaneous C-to-T mutations is decreased by the analog in a dose-dependent manner. (C) 1999 Elsevier Science B.V. All rights reserved.