Increase in TNFα transport after SCI is specific for time, region, and type of lesion
Increase in TNFα transport after SCI is specific for time, region, and type of lesion
复制标题
DOI：
10.1006/exnr.2001.7702
复制
发表时间：
2001-08-01
影响因子：
5.3
通讯作者：
Kastin, AJ
中科院分区：
文献类型：
--
作者：
Pan, WH;Kastin, AJ
血脑屏障和血脊髓屏障(BBB)的动态变化是中枢神经系统损伤反应的重要组成部分。本研究旨在探讨脊髓损伤(SCI)急性期血脑屏障对胸区的通透性。采用加压或完全横断法建立小鼠脊髓损伤模型。通过脊髓摄取放射性标记白蛋白来评估血脑屏障的破坏。胸髓血脑屏障在压迫伤后即刻被破坏，持续2天。随后是损伤后3天开始的颈髓延迟性通透性增加。横断后，血脑屏障的破坏仅限于胸髓，并且仅在损伤后立即出现。肿瘤坏死因子α的进入不仅在血脑屏障破坏时增加，遵循相同的模式，而且在血脑屏障对白蛋白的通透性恢复正常后也有继发性变化。肿瘤坏死因子α进入的增加，最好的解释是肿瘤坏死因子α的特定转运系统上调，在腰髓和胸区明显增加，并且在两种类型的损伤后遵循不同的时间进程。结合我们的结果和文献中关于炎症反应的作用和肿瘤坏死因子α在脊髓再生中的作用的文献，我们得出结论，肿瘤坏死因子α转运上调的时间、区域和损伤特异性是脊髓损伤后血脑屏障调节变化的一部分。(C)2001年学术出版社。
The dynamic changes of the blood-brain barrier and blood-spinal cord barrier (BBB) are an important part of the CNS response to injury. This study addresses the permeability of the BBB in the acute phase of spinal cord injury (SCI) to the thoracic region. SCI by compression or by complete transection was generated in mice. BBB disruption was evaluated by spinal cord uptake of radiolabeled albumin. The BBB of the thoracic spinal cord was disrupted immediately aft compression injury, lasting for 2 days. This was followed by a delayed permeability increase in the cervical spinal cord beginning 3 days after injury. After transection, BBB disruption was limited to the thoracic spinal cord and was present only immediately postinjury. The entry of TNF alpha not only was increased at the time of BBB disruption, following the same pattern, but also had secondary changes after the BBB permeability to albumin had returned to normal. The increase of TNF alpha entry, best explained by upregulation of the specific transport system for TNF alpha, was pronounced in the lumbar spinal cord as well as the thoracic region, and followed a different time course after the two types of injury. Integrating our results with those of the literature regarding the roles of inflammatory responses and the effects of TNF alpha in spinal cord regeneration, we conclude that the time-, region-, and lesion-specificity of the upregulation of TNF alpha transport is part of the regulatory changes at the BBB in response to SCI. (C) 2001 Academic Press.