Phytochrome B regulates reactive oxygen signaling during abiotic and biotic stress in plants
Phytochrome B regulates reactive oxygen signaling during abiotic and biotic stress in plants
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DOI：
10.1111/nph.18626
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发表时间：
2022-12-08
期刊：
影响因子：
9.4
通讯作者：
Mittler, Ron
中科院分区：
文献类型：
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作者：
Fichman, Yosef;Xiong, Haiyan;Mittler, Ron
活性氧 (ROS) 和光感受器蛋白光敏色素 B (phyB) 在植物适应逆境中发挥着关键作用。然而，在应激过程中，主要在细胞核中发挥作用的 phyB 如何影响由存在于质膜上的呼吸爆发氧化酶同系物 (RBOH) 蛋白介导的 ROS 信号转导尚不清楚。拟南芥和水稻突变体、RNA-Seq、生物信息学、生物化学、分子生物学和全植物 ROS 成像被用来解决这个问题。在这里，我们揭示了 phyB 和 RBOH 作为关键调节模块的一部分，控制质外体 ROS 的产生、胁迫反应转录本的表达以及植物对过度光胁迫的适应。我们进一步表明，phyB 可以在应激期间调节 ROS 产生，即使它仅限于细胞质，并且 phyB、呼吸爆发氧化酶蛋白 D (RBOHD) 和呼吸爆发氧化酶蛋白 F (RBOHF) 共同调节数千个转录物以响应光应激。令人惊讶的是，我们发现 phyB 也是响应热、受伤、寒冷和细菌感染时 ROS 积累所必需的。我们的研究结果表明，phyB 在植物对生物和非生物胁迫的反应中发挥着重要作用，可能在细胞质中调节质外体 ROS 的产生，并且 phyB 和 RBOHD/RBOHF 在相同的调节途径中发挥作用。
Reactive oxygen species (ROS) and the photoreceptor protein phytochrome B (phyB) play a key role in plant acclimation to stress. However, how phyB that primarily functions in the nuclei impacts ROS signaling mediated by respiratory burst oxidase homolog (RBOH) proteins that reside on the plasma membrane, during stress, is unknown. Arabidopsis thaliana and Oryza sativa mutants, RNA-Seq, bioinformatics, biochemistry, molecular biology, and whole-plant ROS imaging were used to address this question. Here, we reveal that phyB and RBOHs function as part of a key regulatory module that controls apoplastic ROS production, stress-response transcript expression, and plant acclimation in response to excess light stress. We further show that phyB can regulate ROS production during stress even if it is restricted to the cytosol and that phyB, respiratory burst oxidase protein D (RBOHD), and respiratory burst oxidase protein F (RBOHF) coregulate thousands of transcripts in response to light stress. Surprisingly, we found that phyB is also required for ROS accumulation in response to heat, wounding, cold, and bacterial infection. Our findings reveal that phyB plays a canonical role in plant responses to biotic and abiotic stresses, regulating apoplastic ROS production, possibly while at the cytosol, and that phyB and RBOHD/RBOHF function in the same regulatory pathway.