The teleost cone cytoskeleton. Localization of actin, microtubules, and intermediate filaments.
The teleost cone cytoskeleton. Localization of actin, microtubules, and intermediate filaments.
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DOI：
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发表时间：
1986-05
影响因子：
4.4
通讯作者：
B. Nagle;C. Okamoto;B. Taggart;B. Burnside
中科院分区：
文献类型：
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作者：
B. Nagle;C. Okamoto;B. Taggart;B. Burnside
本实验室一直以硬骨鱼视网膜视锥为模型，研究视网膜细胞运动的机制与调控。在以前的抑制剂研究中，作者已经表明，黑暗诱导的视锥细胞伸长需要微管，而光诱导的收缩需要肌动蛋白丝。本研究探讨了肌动蛋白丝，微管和中间丝的锥细胞骨架的分布。肌动蛋白丝已被定位在孤立的锥体标记与荧光衍生物的鬼笔环肽，微管被定位与抗微管蛋白的免疫荧光标记。肌动蛋白，微管，和中间丝分布也被检查在洗涤剂溶解的运动细胞模型的锥固定的一种新的方法，提高了保存的细胞骨架。肌动蛋白丝的纵向束从锥体的萼突延伸通过椭圆体并进入肌样。在核周区和轴突中检测不到肌动蛋白丝，但在突触的突触前和突触后组分中都存在肌动蛋白丝。中间丝在核周区和锥体轴突中较多，但在肌样中相对稀疏。相比之下，微管分布更均匀：许多纵向取向的微管存在于整个细胞的长度。因此，视锥细胞骨架反映了细胞高度极化的形状和功能，肌动蛋白丝位于细胞的远端可移动部分，中间丝位于细胞的近端部分，其锚定在视网膜中。
This laboratory has been using the teleost retinal cone as a model for studying the mechanisms and regulation of retinal cell motility. In previous inhibitor studies, the authors have shown that dark-induced cone elongation requires microtubules, whereas light-induced contraction requires actin filaments. This study examines the distributions of actin filaments, microtubules, and intermediate filaments in the cone cytoskeleton. Actin filaments have been localized in isolated cones by labeling with fluorescent derivatives of phalloidin; microtubules were localized by immunofluorescent labeling with anti-tubulin. Actin, microtubule, and intermediate filament distributions have also been examined in detergent-lysed motile cell models of cones fixed with a new method that enhances preservation of the cytoskeleton. Longitudinal bundles of actin filaments extend from the cone's calycal processes through the ellipsoid and into the myoid. No actin filaments are detectable in the perinuclear region and axon, but filaments are present in both pre- and post-synaptic components of the synapse. Intermediate filaments are numerous in the perinuclear region and cone axon but relatively sparse in the myoid. In contrast, microtubule distribution is more uniform: numerous longitudinally oriented microtubules are present throughout the length of the cell. Thus the cone cytoskeleton reflects the highly polarized shape and function of the cell, with actin filaments localized to the distal movable part of the cell and intermediate filaments localized to the proximal part of the cell, which is anchored in the retina.