Brassinosteroids control male fertility by regulating the expression of key genes involved in Arabidopsis anther and pollen development
Brassinosteroids control male fertility by regulating the expression of key genes involved in Arabidopsis anther and pollen development
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DOI：
10.1073/pnas.0912333107
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发表时间：
2010-03-30
影响因子：
11.1
通讯作者：
Wang, Xuelu
中科院分区：
文献类型：
--
作者：
Ye, Qianqian;Zhu, Wenjiao;Wang, Xuelu
花药和花粉的发育对雄性生殖是重要的，这一过程受到许多外部和内部线索的协调调控。在这项研究中，我们系统地检测了一系列油菜素类固醇生物合成和信号突变体的雄性生殖表型，发现这些突变体除了预期的细胞扩张缺陷外，还表现出花粉数量、活力和释放效率的降低。这些缺陷与绒毡层和小孢子发育异常有关。通过实时定量RT-PCR和基因芯片实验，我们发现在这些突变体中，花药和花粉发育所需的许多关键基因的表达都受到了抑制。芯片分析表明，BES1是油菜素类固醇信号转导的重要转录因子，可以直接与花药和花粉发育所必需的转录因子SP1/NZZ、TDF1、AMS、MS1和MS2的启动子区域结合。综上所述，这些数据引导我们提出，油菜素类固醇至少部分通过直接调控拟南芥花药和花粉发育的关键基因来控制雄性可育性。我们的工作提供了一种独特的机制来解释植物激素如何调节植物发育所必需的遗传程序。
The development of anther and pollen is important for male reproduction, and this process is coordinately regulated by many external and internal cues. In this study, we systematically examined the male reproductive phenotypes of a series of brassinosteroid biosynthetic and signaling mutants and found that, besides the expected cell-expansion defects, these mutants also showed reduced pollen number, viability, and release efficiency. These defects were related with abnormal tapetum and microspore development. Using both real-time quantitative RTPCR and microarray experiments, we found that the expression of many key genes required for anther and pollen development was suppressed in these mutants. ChIP analysis demonstrated that BES1, an important transcription factor for brassinosteroid signaling, could directly bind to the promoter regions of genes encoding transcription factors essential for anther and pollen development, SPL/NZZ, TDF1, AMS, MS1, and MS2. Taken together, these data lead us to propose that brassinosteroids control male fertility at least in part via directly regulating key genes for anther and pollen development in Arabidopsis. Our work provides a unique mechanism to explain how a phytohormone regulates an essential genetic program for plant development.