Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet.
Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet.
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组合代谢组和转录组学方法方法揭示了对高脂饮食的心脏反应。
DOI：
10.1016/j.isci.2022.104184
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发表时间：
2022-05-20
期刊：
影响因子：
5.8
通讯作者：
Morrow, John P.
中科院分区：
文献类型：
--
作者：
Joseph, Leroy C.;Shi, Jianting;Nguyen, Quynh N.;Pensiero, Victoria;Goulbourne, Chris;Bauer, Robert C.;Zhang, Hanrui;Morrow, John P.
重要器官对不同类型的营养或饮食的反应是生理学中的一个基本问题。我们研究了小鼠对4周高脂饮食的心脏反应，测量心脏代谢物和mRNA。代谢组学显示高脂饮食后的显着差异，包括几个酰基肉毒碱物种的增加。RNA-seq数据显示，这些变化与使用更多脂肪酸作为底物的适应性以及抗氧化蛋白过氧化氢酶的增加一致。mRNA的变化与几个高度反应基因的蛋白质水平的变化相关。我们还发现代谢组学和RNA-seq数据集在基线和高脂肪饮食后都存在显著的性别差异。这项工作揭示了重要器官对代谢组学和转录组学水平的饮食干预的反应，这是生理学中的一个基本问题。这项工作还揭示了心脏代谢物和基因表达的显着性别差异。心脏基因表达在短期高脂饮食后显示适应性变化心脏代谢组学表明高脂饮食后线粒体功能障碍代谢和基因表达在基线时显示显著性别差异高脂饮食引起的变化存在显著性别差异病理生理学;人体代谢;代谢组学;转录组学
The response of vital organs to different types of nutrition or diet is a fundamental question in physiology. We examined the cardiac response to 4 weeks of high-fat diet in mice, measuring cardiac metabolites and mRNA. Metabolomics showed dramatic differences after a high-fat diet, including increases in several acyl-carnitine species. The RNA-seq data showed changes consistent with adaptations to use more fatty acid as substrate and an increase in the antioxidant protein catalase. Changes in mRNA were correlated with changes in protein level for several highly responsive genes. We also found significant sex differences in both metabolomics and RNA-seq datasets, both at baseline and after high fat diet. This work reveals the response of a vital organ to dietary intervention at both metabolomic and transcriptomic levels, which is a fundamental question in physiology. This work also reveals significant sex differences in cardiac metabolites and gene expression. Cardiac gene expression shows adaptive changes after short-term high-fat diet Cardiac metabolomics indicate mitochondrial dysfunction after high-fat diet Metabolites and gene expression show significant sex differences at baseline There are significant sex differences in the changes caused by high-fat diet Pathophysiology; Human metabolism; Metabolomics; Transcriptomics
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DOI：
10.1038/nrm3072
发表时间：
2011-03
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
--
作者：
Love, Michael I;Soneson, Charlotte;Patro, Rob
通讯作者：
Patro, Rob
影响因子：
2
作者：
Okuno, Yosuke;Matsuda, Morihiro;Shimomura, Iichiro
通讯作者：
Shimomura, Iichiro
影响因子：
4.8
作者：
Rindler, Paul M.;Plafker, Scott M.;Kinter, Michael
通讯作者：
Kinter, Michael
影响因子：
64.8
作者：
Sano, Masanori;Minamino, Tohru;Komuro, Issei
通讯作者：
Komuro, Issei