Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells
Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells
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DOI：
10.1128/iai.66.4.1783-1786.1998
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发表时间：
1998-04-01
影响因子：
3.1
通讯作者：
Filler, SG
中科院分区：
文献类型：
--
作者：
Fu, Y;Rieg, G;Filler, SG
为了鉴定介导白色念珠菌与内皮细胞结合的粘附素编码基因，构建了该生物的基因组文库并用于转化酿酒酵母。这些转化的生物体被筛选粘附内皮细胞，并鉴定出高度粘附克隆。该克隆与内皮细胞的粘附性比空质粒转化的酿酒酵母高出100倍以上。该克隆也表现出增强的粘附上皮细胞。该克隆中含有的白色念珠菌基因为ALS1。这些结果表明ALS1可能编码一种候选粘连素。
To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae. These transformed organisms were screened for adherence to endothelial cells, and a highly adherent clone was identified. The adherence of this clone to endothelial cells was over 100-fold greater than that of control S. cerevisiae transformed with the empty plasmid. This clone also exhibited enhanced adherence to epithelial cells. The C. albicans gene contained within this clone was found to be ALS1. These results indicate that ALS1 may encode a candidal adhesin.