Identification of a novel gene, pilZ, essential for type 4 fimbrial biogenesis in Pseudomonas aeruginosa
Identification of a novel gene, pilZ, essential for type 4 fimbrial biogenesis in Pseudomonas aeruginosa
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DOI：
10.1128/jb.178.1.46-53.1996
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发表时间：
1996-01-01
影响因子：
3.2
通讯作者：
Mattick, JS
中科院分区：
文献类型：
--
作者：
Alm, RA;Bodero, AJ;Mattick, JS
机会致病菌铜绿假单胞菌产生4型菌毛，其促进粘附于上皮细胞，并与称为抽搐运动的表面易位形式相关。我们已经使用转座子诱变来鉴定菌毛组装或功能所需的基因座，通过筛选缺乏抽搐运动的扩展菌落形态特征的突变体，这些突变体的一个子集对菌毛特异性噬菌体具有抗性，发现这些突变体之一（R270）在新基因中含有转座子插入，称为pilZ，其位于铜绿假单胞菌图谱上约40 min处的染色体SpeI片段I上，该位置远离参与菌毛生物发生的其它位点。pilZ与编码大肠杆菌DNA聚合酶Ⅲ δ ′亚基的基因holB* 同源，pilZ基因的产物是一个118个氨基酸的蛋白质（预测分子量，12，895），其可能具有细胞质位置，PilZ似乎是一种新的蛋白质，迄今为止还没有在序列数据库中表示，其功能是未知的。互补研究表明，pilZ能够恢复铜绿假单胞菌表面上菌毛的表达，以及当反式提供给R270突变体时，对菌毛特异性噬菌体的抽搐运动性和敏感性。
The opportunistic pathogen Pseudomonas aeruginosa produces type 4 fimbriae which promote adhesion to epithelial cells and are associated with a form of surface translocation called twitching motility. We have used transposon mutagenesis to identify loci required for fimbrial assembly or function by screening for mutants that lack the spreading colony morphology characteristic of twitching motility, A subset of these mutants is resistant to fimbria-specific phage, One of these mutants (R270) was found to contain a transposon insertion in a new gene, termed pilZ, which is located on chromosomal SpeI fragment I at about 40 min on the P. aeruginosa map, a position remote from other loci involved in fimbrial biogenesis. pilZ appears to be linked to and possibly forms an operon with a gene, holB*, which is homologous to the gene encoding the delta' subunit of Escherichia coli DNA polymerase III, The product of the pilZ gene is a protein of 118 amino acids (predicted molecular weight, 12,895) which probably has a cytoplasmic location, PilZ appears to he a new class of protein which has not hitherto been represented in the sequence databases, and its function is unknown. Complementation studies indicate that pilZ is able to restore the expression of fimbriae on the surface of P. aeruginosa as well as twitching motility and sensitivity to fimbria-specific phage when provided in trans to the R270 mutant.