Generation of four iPSC lines from a family harboring a 1p36-35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling
Generation of four iPSC lines from a family harboring a 1p36-35 haplotype linked with bipolar disorder and recurrent depressive disorder: Three-generation patients and a healthy sibling
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来自携带与双相情感障碍和复发性抑郁症相关的 1p36-35 单倍型的家庭的四个 iPSC 系的产生：三代患者和一个健康的兄弟姐妹
DOI：
10.1016/j.scr.2022.102915
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发表时间：
2022
影响因子：
1.2
通讯作者：
Matsushita Masayuki
中科院分区：
文献类型：
--
作者：
Takamatsu Gakuya;Manome Yoko;Lee Jun-Seok;Toyama Kanako;Hayakawa Tomoko;Hara-Miyauchi Chikako;Hasegawa-Ogawa Minami;Katagiri Chiaki;Kondo Tsuyoshi;James Okano Hirotaka;Matsushita Masayuki
从具有遗传特征的患者中获得的诱导多能干细胞（iPSC）有益于双相情感障碍（BD）的生物学研究。在这里，我们介绍了来自BD和复发性抑郁症（RDD）的三代患者和一个家庭中的健康对照兄弟姐妹的iPSC系。所有患者在1 p36 -35中共享指定的单倍型，先前报道为情绪障碍的易感位点。通过非整合的附加型载体用重编程因子0 TC 3/4、l-MYC、LIN 28、S 0X 2、KLF 4和p53 shRNA产生iPSC。所有的iPSC细胞系都强烈表达多能性标记，并证明了体外分化成三个生殖系的能力。
Induced pluripotent stem cells (iPSCs) obtained from genetically characterized patients benefit the biological study of bipolar disorder (BD). Here, we present iPSC lines from three-generation patients with BD and recurrent depressive disorder (RDD) and a healthy control sibling in a family. All patients shared the specified haplotype in the 1p36–35, previously reported as the susceptibility locus of mood disorders. iPSCs were generated with the reprogramming factors OTC3/4,l-MYC, LIN28, SOX2, KLF4, and p53 shRNA through non-integrated episomal vectors. All iPSC lines strongly expressed pluripotency markers and proved the ability to differentiate into three germ lineagesin vitro.