Overexpression of KIF11 in Gastric Cancer with Intestinal Mucin Phenotype
Overexpression of KIF11 in Gastric Cancer with Intestinal Mucin Phenotype
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DOI：
10.1159/000447303
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发表时间：
2016-09-01
期刊：
影响因子：
5
通讯作者：
Yasui, Wataru
中科院分区：
文献类型：
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作者：
Imai, Takeharu;Oue, Naohide;Yasui, Wataru
目的：胃癌是人类最常见的恶性肿瘤之一。一种有用的胃癌干细胞（CSC）的表征方法是球体集落形成。以前，我们报道了与亲本细胞相比，KIF 11在球状体形成GC细胞中的表达>2倍。在此，我们通过免疫组织化学方法分析了KIF 11在人胃癌中的表达和分布。方法：采用免疫组化法检测165例胃癌组织中KIF 11的表达。对于GC的粘蛋白表型表达分析，评价MUC 5AC、MUC 6、MUC 2和CD 10的免疫染色。RNA干扰用于抑制GC细胞系中KIF 11的表达。结果：165例胃癌中，119例（72%）KIF 11阳性。KIF 11的表达与任何临床病理特征无关;然而，在表现出肠道表型的GC中经常观察到。与阴性对照siRNA相比，转染KIF 11靶向siRNA后，MKN-74细胞形成的球体数量和大小均显著减少。此外，磷酸化Erk 1/2的水平在KIF 11 siRNA转染的细胞中低于阴性对照siRNA转染的细胞。结论：KIF 11参与了肠粘蛋白表型GC的表达。(C)2016 S. Karger AG，巴塞尔
Objective: Gastric cancer (GC) is one of the most common human cancers. A useful method of gastric cancer stem cell (CSC) characterization is spheroid colony formation. Previously, we reported that KIF11 expression is >2-fold in spheroid-body-forming GC cells compared with parental cells. Here, we analyzed the expression and distribution of KIF11 in human GC by immunohistochemistry. Methods: Expression of KIF11 in 165 GC cases was determined using immunohistochemistry. For mucin phenotypic expression analysis of GC, immunostaining of MUC5AC, MUC6, MUC2 and CD10 was evaluated. RNA interference was used to inhibit KIF11 expression in GC cell lines. Results: In total, 119 of 165 GC cases (72%) were positive for KIF11. Expression of KIF11 was not associated with any clinicopathologic characteristics; however, it was observed frequently in GC exhibiting an intestinal phenotype. Both the number and size of spheres formed by MKN-74 cells were significantly reduced following transfection of KIF11 -targeting siRNA compared with negative-control siRNA. Furthermore, levels of phosphorylated Erk1/2 were lower in KIF11 siRNA-transfected cells than with negative-control siRNA-transfected cells. Conclusion: These results indicate that KIF11 is involved in intestinal mucin phenotype GC. (C) 2016 S. Karger AG, Basel