Common history at the origin of the position-function correlation in transcriptional regulators in Archaea and Bacteria
Common history at the origin of the position-function correlation in transcriptional regulators in Archaea and Bacteria
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DOI：
10.1007/s002390010207
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发表时间：
2001-09-01
影响因子：
3.9
通讯作者：
Collado-Vides, J
中科院分区：
文献类型：
--
作者：
Pérez-Rueda, E;Collado-Vides, J
具有螺旋-转-螺旋（HTH） DNA结合基序的大肠杆菌中的调节蛋白显示出位置-功能相关性，使得抑制蛋白主要在N端具有该基序，而激活蛋白在c端具有该基序。利用这一初始集合，我们通过序列比较鉴定了17种细菌和6种古细菌基因组中的转录调控因子。这个放大的集合显示了相同的位置-函数相关性。我们要解决的主要问题是，这种相关性是进化中共同起源的结果，还是趋同的结果。证据表明，在这种相关性的起源处有一个共同的历史。我们展示了一个由八个抑制蛋白家族组成的超群的存在，这些家族共享一个保守的扩展序列，包括经典的HTH。其中两个抑制家族（MarR和AsnC）起源于古细菌和细菌分化之前。它们是目前存在于细菌中携带hth的转录调控因子的起源。一组LysR蛋白，HTH也在N端。提供对参数的控制，因为它清楚地显示了独特的结构，功能。，以及进化特性。这组激活蛋白，被认为起源于细菌。有一个有利的基因组织来促进其水平转移-用于征服一些古菌-以及有利于内稳态的负自我调节，所有这些都与细菌中最大的家族一致。这些结果表明，如果基序的洗牌发生在细菌中，它只发生在这些蛋白质历史的早期，而不是在真核生物调节因子中观察到的。
Regulatory proteins in Escherichia coli with a helix-turn-helix (HTH) DNA binding motif show a position-function correlation such that repressors have this motif predominantly at the N terminus, whereas activators have the motif at the C-terminus extreme. Using this initial collection we identified by sequence comparison the exhaustive set of transcriptional regulators in 17 bacterial and 6 archaeal genomes. This enlarged set shows the same position-function correlation. The main question we address is whether this correlation is the result of common origin in evolution or the result of convergence. Evidence is presented supporting a common history at the origin of this correlation. We show the existence of a supergroup of eight repressor protein families sharing a conserved extended sequence comprising the classic HTH. Two of these repressor families (MarR and AsnC) originated before the divergence of Archaea and Bacteria. They are proposed at the origin of HTH-bearing transcriptional regulators currently present in Bacteria. The group of LysR proteins, with the HTH also at the N terminus. offers a control to the argument, since it shows clearly distinctive structural, functional., and evolutionary properties. This group of activator proteins, suggested to have originated within the Bacteria. has an advantageous gene organization to facilitate its horizontal transfer-used to conquer some Archaea-as well as negative autoregulation convenient for homeostasis, all of which agrees with this being the largest family in Bacteria. These results suggest that if shuffling of motifs occurred in Bacteria, it occurred only early in the history of these proteins, as opposed to what is observed in eukaryotic regulators.