Use of fluorescent standards in protein transfer and immunoblotting. Accurate estimation of the molecular weight of immunoreactive species.
Use of fluorescent standards in protein transfer and immunoblotting. Accurate estimation of the molecular weight of immunoreactive species.
复制标题
在蛋白质转移和免疫印迹中使用荧光标准品。
DOI：
10.1016/0003-2697(85)90590-1
复制
发表时间：
1985
影响因子：
2.9
通讯作者：
Lingwood,CA
中科院分区：
文献类型：
--
作者：
Law,H;Lingwood,CA
荧光蛋白分子量标记物已被用于标准化的电转移和随后的免疫染色的蛋白质分离的十二烷基硫酸钠-聚丙烯酰胺电泳。该方法允许准确估计免疫反应性物质的分子量，尽管在转移或染色过程中可能发生收缩或膨胀。
Fluorescent protein molecular weight markers have been used to standardize the electrotransfer and subsequent immunostaining of proteins separated by sodium dodecyl sulfate-polyacrylamide electrophoresis. The method permits accurate estimation of the molecular weight of immunoreactive species despite shrinkage or swelling which may occur during transfer or staining.