Pharmacological characterisation and functional roles for egg-laying of a beta-adrenergic-like octopamine receptor in the brown planthopper Nilaparvata lugens
Pharmacological characterisation and functional roles for egg-laying of a beta-adrenergic-like octopamine receptor in the brown planthopper Nilaparvata lugens
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褐飞虱β-肾上腺素样章鱼胺受体的药理学特征和产卵功能作用
DOI：
10.1016/j.ibmb.2017.06.008
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发表时间：
2017
影响因子：
3.8
通讯作者：
Gao Cong-Fen
中科院分区：
文献类型：
--
作者：
Wu Shun-Fan;Jv Xiao-Min;Li Jian;Xu Guang-Jian;Cai Xiao-Yi;Gao Cong-Fen
章鱼胺，肾上腺素和去甲肾上腺素的无脊椎动物对应物，控制和调节原口动物的许多生理和行为过程。它通过与属于G蛋白偶联受体超家族的特异性受体结合来介导其作用。我们报告的克隆的cDNA从褐飞虱（Nloa2b2）共享高度相似的OA2B2受体类的成员。通过章鱼胺活化N10A2B2以剂量依赖性方式增加cAMP的产生（EC 50 = 114 nM）。酪胺也激活受体，但效力远低于章鱼胺。使用一系列已知的章鱼胺受体的激动剂和拮抗剂以及cAMP测量，我们观察到N10A2B2的相当独特的药理学特征。所测试的激动剂的效力排序为萘甲唑啉>可乐定。与依匹斯汀、米安色林、酚妥拉明、甲硫替平、布他拉莫或麦角新碱共孵育可消除章鱼胺的激活作用。Nloa2b2在不同发育阶段和各种组织中表达，包括已知参与产卵行为的雌性生殖区域。利用活体药理学和RNAi技术，我们证明了N10A2B2信号通路的干扰对褐飞虱雌虫的产卵行为具有强烈的影响。这里提供的数据标志着第一次全面的研究，从基因到行为的OA2B2受体在水稻褐飞虱。
Octopamine, the invertebrate counterpart of adrenaline and noradrenaline, controls and modulates many physiological and behavioral processes in protostomes. It mediates its effects by binding to specific receptors belonging to the superfamily of G-protein coupled receptors. We report the cloning of a cDNA from the brown planthopper (Nloa2b2) sharing high similarity with members of the OA2B2 receptor class. Activation of NlOA2B2 by octopamine increased the production of cAMP in a dose-dependent manner (EC50= 114 nM). Tyramine also activated the receptor but with much less potency than octopamine. Using a series of known agonists and antagonists of octopamine receptors and cAMP measurements, we observed a rather unique pharmacological profile of NlOA2B2. The potency ranking of the tested agonists was naphazoline > clonidine. The activated effect of octopamine is abolished by co-incubation with epinastine, mianserin, phentolamine, methiothepin, butaclamol or methysergide.Nloa2b2was expressed in different developmental stages and in various tissues including female reproductive regions known to be involved in egg-laying behavior. Usingin vivopharmacology and RNAi methodology, we demonstrated that interference of NlOA2B2 signaling pathway had a strong impact on the egg-laying behavior of female brown planthopper. The data presented here mark the first comprehensive study—from gene to behavior—of a OA2B2 receptor in the rice brown planthopper.