Correlation Between Compositional and Mechanical Properties of Human Mesenchymal Stem Cell-Collagen Microspheres During Chondrogenic Differentiation
Correlation Between Compositional and Mechanical Properties of Human Mesenchymal Stem Cell-Collagen Microspheres During Chondrogenic Differentiation
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DOI：
10.1089/ten.tea.2010.0078
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发表时间：
2011-03-01
影响因子：
4.1
通讯作者：
Chan, Barbara P.
中科院分区：
文献类型：
--
作者：
Li, Chun-hei;Chik, Tsz-Kit;Chan, Barbara P.
基于间充质干细胞（MSC）的工程技术在软骨修复方面很有前景。然而，鉴于这种成分力学关系在天然软骨组织中的重要性，工程化结构的成分力学关系尚未得到广泛研究。在本研究中，使用一种新型的人MSC - 胶原蛋白微球系统，通过组织学、生物化学方法以及微孔板压缩试验，研究体外软骨形成分化过程中的成分力学关系。发现力学性能与新沉积的软骨相关基质、糖胺聚糖和II型胶原蛋白以及胶原蛋白交联剂密度呈正相关，这与结构表征中存在的粗大胶原束相符。另一方面，力学性能与I型胶原蛋白和总胶原蛋白呈负相关，这表明微球系统的初始胶原蛋白基质支架正在被分化的人MSC重塑。本研究还展示了一种简单、灵敏且无损的工具在监测组织工程构建物中间充质干细胞软骨形成分化进程中的应用，从而有助于未来新型软骨修复策略的开发。
Mesenchymal stem cell (MSC)-based engineering is promising for cartilage repair. However, the compositional mechanical relationship of the engineered structures has not been extensively studied, given the importance of such relationship in native cartilage tissues. In this study, a novel human MSC-collagen microsphere system was used to study the compositional mechanical relationship during in vitro chondrogenic differentiation using histological and biochemical methods and a microplate compression assay. The mechanical property was found positively correlating with newly deposited cartilage-relevant matrices, glycosaminoglycan, and type II collagen, and with the collagen crosslinker density, in agreement with the presence of thick collagen bundles upon structural characterization. On the other hand, the mechanical property negatively correlates with type I collagen and total collagen, suggesting that the initial collagen matrix scaffold of the microsphere system was being remodeled by the differentiating human MSCs. This study also demonstrated the application of a simple, sensitive, and nondestructive tool for monitoring the progression of chondrogenic differentiation of MSCs in tissue-engineered constructs and therefore contributes to future development of novel cartilage repair strategies.