Effect of transport conditions on the stability of biochemical markers in blood.
Effect of transport conditions on the stability of biochemical markers in blood.
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DOI：
10.1093/clinchem/35.12.2313
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发表时间：
1989-12
影响因子：
9.3
通讯作者：
S. Hankinson;Stephanie J. London;Christopher G. Chute;Robert L. Barbieri;Loring Jones;L. Kaplan;Frank M. Sacks;M. Stampfer
中科院分区：
文献类型：
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作者：
S. Hankinson;Stephanie J. London;Christopher G. Chute;Robert L. Barbieri;Loring Jones;L. Kaplan;Frank M. Sacks;M. Stampfer
我们检查了由邮寄到中心地点进行处理的全血制备的血浆中脂质、类胡萝卜素、α-生育酚和内源激素的稳定性。最初，为了模拟运输条件，全血样本在处理前在实验室中在室温或冷却下保存长达 72 小时。在后面的样本中，脂质浓度每天变化高达 1.4%，类胡萝卜素变化高达 -5.5%，激素浓度变化高达 9.5%。在第二项研究中，将通过隔夜快递邮寄的冷却全血血浆中的分析物浓度与立即分离、冷冻并通过隔夜快递邮寄的血浆中的分析物浓度进行了比较。胆固醇、高密度脂蛋白亚组分 3、载脂蛋白 B 和视黄醇的浓度稳定。总体而言，对于除雌二醇之外的每个标记物，人与人之间的变异至少是人内变异的两倍。在第三项研究中，从 30 mL 冷藏运输的全血中回收了至少 340 微克 DNA。我们的结果表明，通过隔夜快递运送全血样本对于流行病学研究中感兴趣的几种生化标记物的测定是可行的。
We examined the stability of lipids, carotenoids, alpha-tocopherol, and endogenous hormones in plasma prepared from whole blood that had been mailed to a central location for processing. Initially, to simulate transport conditions, whole-blood samples were stored in the laboratory, either at room temperature or cooled, for up to 72 h before processing. In the latter samples, lipid concentrations changed up to 1.4% per day, carotenoids up to -5.5%, and hormones up to 9.5%. In a second study, analyte concentrations in plasma from cooled whole blood mailed via overnight courier were compared with those from plasma that had been immediately separated, frozen, and mailed via overnight courier. Concentrations of cholesterol, high-density lipoprotein subfraction 3, apolipoprotein B, and retinol were stable. Overall, for each marker except estradiol, the between-person variation was at least twice the within-person variation. In a third study, at least 340 micrograms of DNA was recovered from 30 mL of cool-shipped whole blood. Our results indicate that shipping whole-blood samples by overnight courier is feasible for assay of several biochemical markers of interest in epidemiological research.