Responses of isolated pulmonary arteries to synthetic peptide F-Met-Leu-Phe.
Responses of isolated pulmonary arteries to synthetic peptide F-Met-Leu-Phe.
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离体肺动脉对合成肽 F-Met-Leu-Phe 的反应。
DOI：
10.1152/ajpheart.1989.257.1.h107
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Reed,WP
中科院分区：
文献类型：
--
作者：
Crowell,RE;VanEpps,DE;Reed,WP
化学引诱物甲酰甲硫氨酸亮氨酸苯丙氨酸（FMLP）最近已被证明具有痉挛的性质，在平滑肌制剂从各种器官。在这项研究中，我们研究了这种肽对离体兔肺动脉（PA）环段的作用。在静息张力下，FMLP刺激PA的浓度依赖性收缩。然而，在PA已预收缩去甲肾上腺素，FMLP刺激浓度依赖性舒张。FMLP刺激的PA收缩抑制早期暴露于吲哚美辛或furegrelate，血栓素合成酶抑制剂，但不是由早期暴露于H1组胺受体拮抗剂pyrilamine。FMLP刺激的PA松弛完全取消吲哚美辛，但不是由furegrelate或pyrilamine。PA制剂中内皮的破坏降低了对肽的收缩和舒张反应，表明这些细胞参与了这些反应。这些结果表明，趋化因子FMLP可以引起孤立PA的收缩或松弛，这取决于潜在的活性PA张力。此外，收缩和舒张都依赖于环氧合酶产物和完整的内皮。
The chemoattractant formyl-methionine-leucine-phenylalanine (FMLP) has recently been shown to possess spasmogenic properties in smooth muscle preparations from various organs. In this study we have investigated the actions of this peptide on isolated rabbit pulmonary artery (PA) ring segments. FMLP stimulated concentration-dependent constriction of PA at resting tension. However, in PA that had been preconstricted by norepinephrine, FMLP stimulated concentration-dependent relaxation. FMLP-stimulated PA constriction was inhibited by earlier exposure to indomethacin or to furegrelate, a thromboxane synthetase inhibitor, but not by earlier exposure to the H1 histamine receptor antagonist pyrilamine. FMLP-stimulated relaxation of PA was totally abolished by indomethacin but not by furegrelate or pyrilamine. Disruption of the endothelium in PA preparations decreased both the constriction and relaxation response to the peptide, suggesting that these cells were involved in these responses. These results indicate that the chemotactic factor FMLP can elicit constriction or relaxation of isolated PA, depending on the underlying active PA tension. In addition, both constriction and relaxation are dependent on cyclooxygenase products and intact endothelium.