Orientation of collagen at the osteocyte lacunae in human secondary osteons
Orientation of collagen at the osteocyte lacunae in human secondary osteons
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DOI：
10.1016/j.jbiomech.2008.09.010
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发表时间：
2008-12-05
影响因子：
2.4
通讯作者：
Lomovtsev, Alexander
中科院分区：
文献类型：
--
作者：
Ascenzi, Maria-Grazia;Gill, Jaya;Lomovtsev, Alexander
这项工作的一个方面的人骨微结构的特点，有关骨折的启动和逮捕。它解决了如何接近骨细胞陷窝的基本组件的方向影响次级骨的微观生物力学。新的数据在骨关节腔周围区域的胶原-磷灰石的方向，和以前的数据外的骨关节腔周围区域的胶原取向，被纳入一个新的模拟骨单位，研究如何在机械测试过程中的应变和应力的方向。共聚焦显微镜观察从骨单位分离的单个板层标本中的骨陷窝周围区域。在圆偏振光下，通过横截面上的熄灭或明亮外观分离样品。这是因为同步加速器衍射和共聚焦显微镜已经确定，每种类型，远离月旁区域，对应于特定的主导胶原蛋白取向（灭绝lamelae的主导胶原蛋白与原始骨单位轴形成小角度，而明亮lamelae的形成更大的角度）。每个骨陷窝周围区域的连续共聚焦图像的形态测量显示胶原蛋白的方向通常遵循小管的方向，环绕垂直于骨陷窝。相对于片层壁倾斜的陷窝在绝灭片层中比在亮片层中多。它们的顶端不太可能在熄灭比明亮的板层显示胶原蛋白以下小管方向。模拟骨单位中骨细胞陷窝，在拉伸或压缩载荷下，支持胶原蛋白取向影响应变和应力的假设，在赤道周围的陷窝的存在结合。我们进一步推测，胶原蛋白的取向转移的传播微裂纹从顶点开始。(C)2008爱思唯尔有限公司保留所有权利。
This work characterizes an aspect of human bone micro-structure, pertinent to fracture initiation and arrest. It addresses how the orientation of elementary components proximate to osteocyte lacunae influences secondary osteon micro-biomechanics. New data at the perilacunar region concerning orientation of collagen-apatite, and prior data on collagen Orientation outside the perilacunar region, are incorporated in a novel Simulation of osteons to investigate how orientation relates to strains and stresses during mechanical testing. The perilacunar region was observed by confocal microscopy within single lamellar specimens, isolated from osteons. The specimens were separated by extinct or bright appearance in transverse section under circularly polarizing light. This is because Synchrotron diffraction and confocal microscopy had established that each type, away from the perilacunar region, corresponds to specific dominant collagen orientation (extinct lamellae's dominant collagen forming small angles with the original osteon axis, while the bright lamellae's forms larger angles). Morphometry of serial confocal images of each perilacunar region showed collagen orientation generally following the orientation of canaliculi, circumambiently-perpendicular to the lacuna. The lacunae tilted relative to the lamellar walls were more numerous in extinct than in bright lamella. Their apices were less likely in extinct than bright lamella to show collagen following the canalicular orientation. The simulation of osteocyte lacunae in osteons, under tension or compression loading, Supports the hypothesis that collagen orientation affects strains and stresses at the equatorial perilacunar region in conjunction with the presence of the lacuna. We further conjecture that collagen orientation diverts propagation of micro-cracks initiating from apices. (C) 2008 Elsevier Ltd. All rights reserved.