Dedifferentiated fat cells in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration
Dedifferentiated fat cells in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration
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DOI：
10.1016/j.reth.2019.08.004
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发表时间：
2019-12-01
影响因子：
4.3
通讯作者：
Sakurai, Hiroyuki
中科院分区：
文献类型：
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作者：
Fujimaki, Hiroshi;Matsumine, Hajime;Sakurai, Hiroyuki
简介：聚乙醇酸（PGA）神经导管是目前应用于临床的一种人工可生物降解神经再生诱导管，可有效促进周围神经再生。当在适当的分化诱导条件培养基中培养时，去分化脂肪（DFAT）细胞分化成各种细胞，包括脂肪细胞、成骨细胞、软骨细胞、骨骼肌细胞和肌成纤维细胞。本研究通过在PGA导管中填充DFAT细胞制作混合型人工神经导管，并将其应用于大鼠面神经缺损模型，并考察导管的面神经再生能力。方法：吸入麻醉下，暴露Lewis大鼠面神经颊支，制造7 mm的神经缺损。 PGA神经导管内填充DFAT细胞，以大鼠皮下脂肪组织为原料，以I型胶原为支架制备，然后在显微镜下移植到大鼠神经缺损部位（DFAT组）（n = 10）。在其他大鼠中，仅将 PGA 人工神经导管类似地移植到神经缺损部位（对照组）（n = 10）。术后13周通过逆行示踪剂证实神经支配重新存在，随后进行组织学和生理学比较研究。结果：DFAT组有髓纤维的平均数量（1605+/-806.23）显着高于对照组（543.6+/-478.66）。 DFAT组的髓磷脂厚度(0.57+/-0.17μm)也显着大于对照组(0.46+/-0.14μm)。尽管DFAT组（2.84±2.47mV）和对照组（0.88±0.56mV）之间复合肌肉动作电位（CMAP）幅度没有发现显着差异，但DFAT组（9.22度+/-0.65度）的胡须运动大于对照组（1.9度+/-0.84度）。结论：发现了DFAT细胞填充的PGA导管促进实验性大鼠面神经缺损模型中的神经再生。 (C) 2019，日本再生医学会。由 Elsevier B.V. 制作和主持
Introduction: Polyglycolic acid (PGA) nerve conduits, an artificial biodegradable nerve regeneration-inducing tube currently used in clinical practice, are effective in regenerating peripheral nerves. Dedifferentiated fat (DFAT) cells differentiate into various cells including adipocytes, osteoblasts, chondrocytes, skeletal muscle cells, and myofibroblasts, when cultured in appropriate differentiation-inducing conditioned culture medium. This study made a hybrid artificial nerve conduit by filling a PGA conduit with DFAT cells, applied the conduit to a rat facial nerve defect model, and investigated the facial nerve regenerative ability of the conduit.Methods: Under inhalational anesthesia, the buccal branch of the facial nerve in Lewis rats was exposed, and a 7-mm nerve defect was created. PGA nerve conduits were filled with DFAT cells, which were prepared from rat subcutaneous adipose tissue with type I collagen as a scaffold, and then grafted into the nerve defect sites in rats with a microscope (DFAT group) (n = 10). In other rats, PGA artificial nerve conduits alone were similarly grafted into the nerve defect sites (the control group) (n = 10). Reinnervation was confirmed at 13 weeks postoperatively by a retrograde tracer, followed by histological and physiological comparative studies.Results: The mean number of myelinated fibers was significantly higher in DFAT group (1605 +/- 806.23) than in the control group (543.6 +/- 478.66). Myelin thickness was also significantly lager in DFAT group (0.57 +/- 0.17 mu m) than in the control group.(0.46 +/- 0.14 mu m). Although no significant difference was found in the amplitude of compound muscle action potential (CMAP) between DFAT group (2.84 +/- 2.47 mV) and the control group (0.88 +/- 0.56 mV), whisker motion was lager in DFAT group (9.22 degrees +/- 0.65 degrees) than in the control group (1.9 degrees +/- 0.84 degrees).Conclusions: DFAT cell-filled PGA conduits were found to promote nerve regeneration in an experimental rat facial nerve defect model. (C) 2019, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.