Flavonoids in Ageratum conyzoides L. Exert Potent Antitumor Effects on Human Cervical Adenocarcinoma HeLa Cells In Vitro and In Vivo
Flavonoids in Ageratum conyzoides L. Exert Potent Antitumor Effects on Human Cervical Adenocarcinoma HeLa Cells In Vitro and In Vivo
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DOI：
10.1155/2020/2696350
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发表时间：
2020-05-04
影响因子：
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通讯作者：
Hou, Youming
中科院分区：
文献类型：
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作者：
Lin, Zeyan;Lin, Yanyan;Hou, Youming
Ageratum conyzoides L.（A. conyzoides）通常用作传统药物，其抗肿瘤作用也已被研究。然而，A. conyzoides 中黄酮类化合物在抗肿瘤活性中的功能作用尚未阐明。本研究旨在研究 A. conyzoides 中的黄酮类化合物对人宫颈腺癌的生物学效应。首先，我们在体外检测到A. conyzoides中的黄酮类化合物显着抑制人宫颈腺癌HeLa细胞的增殖、侵袭、迁移和克隆性。此外，我们发现A. conyzoides中的黄酮类化合物可诱导HeLa细胞显着的S期阻滞和细胞凋亡，并明显降低细胞内活性氧（ROS）水平。最后，我们发现A. conyzoides中的黄酮类化合物显着抑制体内HeLa异种移植肿瘤的生长和上皮间质转化（EMT）。总之，我们的结果证明了A. conyzoides中的黄酮类化合物对HeLa细胞具有明显的抗肿瘤作用，这表明A. conyzoides中的黄酮类化合物可以作为人类宫颈腺癌的新型治疗化合物。
The Ageratum conyzoides L. (A. conyzoides) is commonly used as a traditional medicine, and its antitumor effects have also been studied. However, the functional roles of flavonoids in A. conyzoides in antitumor activities have not been clarified. The present study is aimed at investigating the biological effects of flavonoids in A. conyzoides on human cervical adenocarcinoma. Firstly, we detected that flavonoids in A. conyzoides significantly inhibited the proliferation, invasion, migration, and clonality of human cervical adenocarcinoma HeLa cells in vitro. Furthermore, we found that flavonoids in A. conyzoides induced significant S phase arrest and apoptosis and obviously decreased the intracellular reactive oxygen species (ROS) level in HeLa cells. Finally, we found that flavonoids in A. conyzoides significantly inhibited the HeLa xenograft tumor growth and epithelial-mesenchymal transition (EMT) in vivo. In conclusion, our results demonstrated the obvious antitumor effects of flavonoids in A. conyzoides on HeLa cells, suggesting that flavonoids in A. conyzoides could be provided as a novel therapeutic compound for human cervical adenocarcinoma.