Tetra primer ARMS-PCR for identification of SNP in β-tubulin of Botrytis cinerea, responsible of resistance to benzimidazole
Tetra primer ARMS-PCR for identification of SNP in β-tubulin of Botrytis cinerea, responsible of resistance to benzimidazole
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DOI：
10.1016/j.mimet.2009.06.007
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发表时间：
2009-08-01
影响因子：
2.2
通讯作者：
Lanza Volpe, Melisa
中科院分区：
文献类型：
--
作者：
Munoz, Claudio;Gomez Talquenca, Sebastian;Lanza Volpe, Melisa
采用竞争性聚合酶链反应（Tetra primer amplification refractory mutation system，ARMS）技术对灰葡萄孢（Botrytis cinerea）β-微管蛋白基因的单核苷酸多态性（single nucleotide polymorphism，SNP）进行了分析。在分析的35个分离株中，其中6个在该基因中显示出SNP，这很容易通过该技术鉴定，并且在6个病例中对应于耐药菌株。(c)2009 Elsevier B. V.保留所有权利。
A competitive polymerase chain reaction (PCR) technique called Tetra primer amplification refractory mutation system (ARMS) PCR was adapted to identify a single nucleotide polymorphism (SNP) in beta-tubulin gene of Botrytis cinerea. Over the 35 isolates analyzed, six of them showed a SNP in that gene, which was readily identified by the technique and in the six cases correspond to the resistant strain. (c) 2009 Elsevier B.V. All rights reserved.