Electrophysiological characterization of neurons in the dorsolateral pontine rapid-eye-movement sleep induction zone of the rat: Intrinsic membrane properties and responses to carbachol and orexins
Electrophysiological characterization of neurons in the dorsolateral pontine rapid-eye-movement sleep induction zone of the rat: Intrinsic membrane properties and responses to carbachol and orexins
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DOI：
10.1016/j.neuroscience.2006.08.045
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发表时间：
2006-12-13
期刊：
影响因子：
3.3
通讯作者：
McCarley, R. W.
中科院分区：
文献类型：
--
作者：
Brown, R. E.;Winston, S.;McCarley, R. W.
药理学，病变和单单位记录技术在几种动物已经确定了一个区域的脑桥网状结构（subcoeruleus，SubC）腹侧的蓝斑作为关键参与快速眼动（REM）睡眠的产生。然而，SubC神经元的内在膜特性和反应，重要的REM睡眠控制，如乙酰胆碱和食欲素/hypocretins神经递质，以前没有在任何动物物种进行过研究，因此在本study.We的目标是获得全细胞膜片钳记录从视觉识别SubC神经元在体外大鼠脑切片。两组大的神经元（平均直径30和27 μ m）被初步确定为胆碱能（吻侧SubC）和去甲肾上腺素能（尾侧SubC）神经元。SubC网状神经元（非胆碱能、非去甲肾上腺素能）在超极化电流脉冲过程中表现出中等大小的去极化凹陷，并经常出现反弹去极化（低阈值尖峰，LTS）。在去极化电流脉冲，他们表现出很小的适应和发射最大在30-90赫兹。这些SubC网状神经元兴奋卡巴胆碱（n=27）自发发射6 Hz，往往表现出中等大小的LTS，并广泛的大小（17-42 μ m）变化。卡巴胆碱抑制SubC网状神经元是中等大小（15-25 μ m），并构成两组。较大的组（n=22）在休息时是安静的，并具有突出的LTS和相关的1至4个动作电位。第二个较小的组（n=8）在超极化脉冲的偏移处延迟返回基线。食欲素对卡巴胆碱兴奋和卡巴胆碱抑制的SubC网状神经元都有兴奋作用，SubC网状神经元的固有膜特性和对卡巴胆碱的反应与其他网状神经元相似，但发现了大量卡巴胆碱抑制的神经元（> 50%），其中大多数表现出明显的LTS，可能对应于桥脑-膝状体-枕叶爆发神经元。一些或所有卡巴胆碱兴奋的神经元可能是REM-on神经元。(c)2006年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Pharmacological, lesion and single-unit recording techniques in several animal species have identified a region of the pontine reticular formation (subcoeruleus, SubC) just ventral to the locus coeruleus as critically involved in the generation of rapid-eye-movement (REM) sleep. However, the intrinsic membrane properties and responses of SubC neurons to neurotransmitters important in REM sleep control, such as acetylcholine and orexins/hypocretins, have not previously been examined in any animal species and thus were targeted in this study.We obtained whole-cell patch-clamp recordings from visually identified SubC neurons in rat brain slices in vitro. Two groups of large neurons (mean diameter 30 and 27 mu m) were tentatively identified as cholinergic (rostral SubC) and noradrenergic (caudal SubC) neurons. SubC reticular neurons (non-cholinergic, non-noradrenergic) showed a medium-sized depolarizing sag during hyperpolarizing current pulses and often had a rebound depolarization (low-threshold spike, LTS). During depolarizing current pulses they exhibited little adaptation and fired maximally at 30-90 Hz. Those SubC reticular neurons excited by carbachol (n=27) fired spontaneously at 6 Hz, often exhibited a moderately sized LTS, and varied widely in size (17-42 mu m). Carbachol-inhibited SubC reticular neurons were medium-sized (15-25 mu m) and constituted two groups. The larger group (n=22) was silent at rest and possessed a prominent LTS and associated one to four action potentials. The second, smaller group (n=8) had a delayed return to baseline at the offset of hyperpolarizing pulses. Orexins excited both carbachol excited and carbachol inhibited SubC reticular neurons.SubC reticular neurons had intrinsic membrane properties and responses to carbachol similar to those described for other reticular neurons but a larger number of carbachol inhibited neurons were found (> 50%), the majority of which demonstrated a prominent LTS and may correspond to Pontine-geniculate-occipital burst neurons. Some or all carbachol-excited neurons are presumably REM-on neurons. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.