Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae
Bro1 is an endosome-associated protein that functions in the MVB pathway in Saccharomyces cerevisiae
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DOI：
10.1242/jcs.00395
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发表时间：
2003-05-15
影响因子：
4
通讯作者：
Emr, SD
中科院分区：
文献类型：
--
作者：
Odorizzi, G;Katzmann, DJ;Emr, SD
多泡体是晚期内体区室，含有由限制性内体膜向内出芽形成的内腔小泡。在酿酒酵母中，完整的膜蛋白被分选到多泡体的内腔囊泡中，并且该过程需要VPS基因的E类子集。我们发现，E类VPS基因之一，BRO 1/VPS 31，编码一种细胞质蛋白，与内体隔间。Bro 1从内体解离需要另一种E类Vps蛋白Vps 4，其是一种ATP酶，也调节ESCRT-III的内体解离，ESCRT-III是四种E类Vps蛋白（Vps 2、Vps 20、Vps 24和Snf 7/Vps 32）的复合物，其在内体膜处寡聚。我们还表明，内体协会的Bro 1是专门依赖于ESCRT-III的组件之一，Snf 7。我们的数据表明，Bro 1在MVB通路中的功能发生在内体膜上，并与ESCRT-III复合物的功能一致或在其下游发生。
Multivesicular bodies are late endosomal compartments containing lumenal vesicles that are formed by inward budding of the limiting endosomal membrane. In the yeast Saccharomyces cerevisiae, integral membrane proteins are sorted into the lumenal vesicles of multivesicular bodies, and this process requires the class E subset of VPS genes. We show that one of the class E VPS genes, BRO1/VPS31, encodes a cytoplasmic protein that associates with endosomal compartments. The dissociation of Bro1 from endosomes requires another class E Vps protein, Vps4, which is an ATPase that also regulates the endosomal dissociation of ESCRT-III, a complex of four class E Vps proteins (Vps2, Vps20, Vps24 and Snf7/Vps32) that oligomerize at the endosomal membrane. We also show that the endosomal association of Bro1 is specifically dependent on one of the ESCRT-III components, Snf7. Our data suggest that the function of Bro1 in the MVB pathway takes place on endosomal membranes and occurs in concert with or downstream of the function of the ESCRT-III complex.