Assessment of organ culture for the conservation of human skin allografts
Assessment of organ culture for the conservation of human skin allografts
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DOI：
10.1007/s10561-007-9042-3
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发表时间：
2008-03-01
影响因子：
1.5
通讯作者：
Magalon, G.
中科院分区：
文献类型：
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作者：
Hautier, A.;Sabatier, F.;Magalon, G.
目的人同种异体皮肤移植是治疗严重烧伤的重要手段，其保存是保证临床疗效的关键。目前的保存方法，如4 ℃保存或冷冻保存，不能防止组织活力的下降。本研究的目的是评估一种新的皮肤器官培养模型中的皮肤同种异体移植物的生存能力和功能，使保护参数尽可能接近生理条件：32摄氏度，气液界面和生理皮肤tension.Design十二个皮肤样本，收获6个活的手术供体，保存35天，在两种条件：保护在4摄氏度和器官培养。在第0、7、14、21、28和35天，使用细胞培养方法（台盼蓝拒染法、集落形成效率和生长速率）、组织病理学和组织酶学研究（Ki67免疫染色）对皮肤样品的活力和功能进行了研究。两种情况之间未观察到统计学差异。两种方法在成纤维细胞和角质形成细胞培养参数的改变方面也相当，与新鲜皮肤相比，这两种方法在第7天和第21天分别显著降低。相比之下，组织病理学和histoenzymological研究显示，更好的保护皮肤的结构和增殖潜力在4摄氏度，相比器官culture.Conclusion这些结果表明，皮肤器官培养不提供显着的优势，皮肤同种异体移植保存。然而，其作为研究皮肤生理学和伤口愈合的实验模型的潜在用途应进一步评估。
Objective Human skin allografts are used in the treatment of severe bums and their preservation is therefore critical for optimal clinical benefit. Current preservation methods, such as 4 degrees C storage or cryopreservation, cannot prevent the decrease of tissue viability. The aim of this study was to assess viability and function of skin allografts in a new skin organ culture model, allowing conservation parameters as close as possible to physiological conditions: 32 degrees C, air-liquid interface and physiological skin tension.Design Twelve skin samples, harvested from 6 living surgical donors, were conserved 35 days in two conditions: conservation at 4 degrees C and organ culture. Viability and function of skin samples were investigated at Day 0, 7, 14, 21, 28 and 35 using cell culture methods (trypan blue exclusion, Colony Forming Efficiency and Growth Rate), histopathological and histoenzymological studies (Ki67 immunostaining).Results In the two conditions, fibroblast and keratinocyte viability was progressively affected by storage, with a significant decrease observed after 35 days. No statistical difference could be observed between the two conditions. The two methods were also comparable regarding alterations of fibroblast and keratinocyte culture parameters, which were respectively significantly reduced at Day 7 and 21, compared to fresh skin. By contrast, histopathological and histoenzymological studies revealed a better preservation of skin architecture and proliferative potential at 4 degrees C, as compared to organ culture.Conclusion These results indicate that skin organ culture does not provide significant advantages for skin allograft preservation. However, its potential use as an experimental model to study skin physiology and wound healing should be further evaluated.