Differential Gene Expression in Granulosa Cells from Polycystic Ovary Syndrome Patients with and without Insulin Resistance: Identification of Susceptibility Gene Sets through Network Analysis
Differential Gene Expression in Granulosa Cells from Polycystic Ovary Syndrome Patients with and without Insulin Resistance: Identification of Susceptibility Gene Sets through Network Analysis
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DOI：
10.1210/jc.2011-3441
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发表时间：
2012-10-01
影响因子：
5.8
通讯作者：
Singh, Rita
中科院分区：
文献类型：
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作者：
Kaur, Surleen;Archer, Kellie J.;Singh, Rita
背景：多囊卵巢综合征 (PCOS) 是一种异质性、遗传复杂性、病因不明的女性内分泌疾病。目的：我们的目的是比较有或没有胰岛素抵抗的 PCOS 女性与匹配对照的受刺激颗粒细胞的基因表达谱。研究设计和方法：本研究包括 12 名正常排卵女性（对照）和 12 名没有胰岛素证据的 PCOS 女性 抵抗（PCOS 非-IR），以及 16 名患有胰岛素抵抗（PCOS-IR）的女性接受体外受精。使用 Affymetrix Human Genome-U133 阵列完成颗粒细胞基因表达谱分析。使用独创性路径分析工具根据基因本体对差异表达基因进行分类。通过实时定量 PCR 确认所选基因的微阵列结果。结果：PCOS non-IR 和 PCOS-IR 颗粒细胞中共有 211 个基因差异表达（倍数变化 >= 1.5；P
Context: Polycystic ovary syndrome (PCOS) is a heterogeneous, genetically complex, endocrine disorder of uncertain etiology in women.Objective: Our aim was to compare the gene expression profiles in stimulated granulosa cells of PCOS women with and without insulin resistance vs. matched controls.Research Design and Methods: This study included 12 normal ovulatory women (controls), 12 women with PCOS without evidence for insulin resistance (PCOS non-IR), and 16 women with insulin resistance (PCOS-IR) undergoing in vitro fertilization. Granulosa cell gene expression profiling was accomplished using Affymetrix Human Genome-U133 arrays. Differentially expressed genes were classified according to gene ontology using ingenuity pathway analysis tools. Microarray results for selected genes were confirmed by real-time quantitative PCR.Results: A total of 211 genes were differentially expressed in PCOS non-IR and PCOS-IR granulosa cells (fold change >= 1.5; P