A spiroplasma of serogroup IV causes a May-disease-like disorder of honeybees in Southwestern France
A spiroplasma of serogroup IV causes a May-disease-like disorder of honeybees in Southwestern France
复制标题
法国西南部的蜜蜂中，IV 群螺原体引起类似五月病的疾病
作者：
C. Mouchès;J. Bové;J. Albisetti;T. B. Clark;J. Tully
在法国西南部，蜜蜂感染了一种类似于经典的“五月病”的疾病，在它们的消化道和血淋巴中含有许多螺旋状的能动生物。在BSR螺原体培养基中培养了两种菌株（B31和B39）并进行了三次克隆。螺旋体蛋白在1 -和2-维聚丙烯酰胺凝胶电泳模式相似的第IV组螺旋体F1和F2，培养以前从花表面在法国。该生物体可在羽化后的不同阶段通过注射或摄食引入成年蜜蜂。通过任一途径施用的药剂在血淋巴中倍增至高滴度并杀死蜜蜂。四环素能抑制该菌的增殖和诱导致死作用，青霉素则不能。与B31和B39菌株几乎相同的螺旋体也从蜜蜂从患病蜂巢访问的区域内收集的花的表面回收。
Honeybees affected by a disorder resembling the classical “May disease” in southwestern France contained numerous helical, motile organisms in their digestive tracts and hemolymph. Two strains of the organism (B31 and B39) were cultured and triply cloned in the BSR spiroplasma medium. The electrophoretic patterns of spiroplasmal proteins in 1 - and 2-dimensional polyacrylamide gels were similar to those of group IV spiroplasmas F1 and F2, cultured previously from flower surfaces in France. The organism could be introduced into adult bees by injection or food ingestion at various stages after emergence. Agent administered by either route multiplied to high titers in the hemolymph and killed the bees. Both multiplication and the induced lethal effect of the agent could be prevented by tetracycline but not penicillin. Spiroplasmas that were nearly identical to the B31 and B39 strains were also recovered from the surface of flowers collected within the area visited by the bees from the diseased hives.