From cutting edge to guideline: A first step in harmonization of the zebrafish embryotoxicity test (ZET) by describing the most optimal test conditions and morphology scoring system
From cutting edge to guideline: A first step in harmonization of the zebrafish embryotoxicity test (ZET) by describing the most optimal test conditions and morphology scoring system
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DOI：
10.1016/j.reprotox.2015.06.050
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发表时间：
2015-08-15
影响因子：
3.3
通讯作者：
Emmen, Harry
中科院分区：
文献类型：
--
作者：
Beekhuijzen, Manon;de Koning, Coco;Emmen, Harry
在过去的几年里，人们对斑马鱼胚胎毒性试验(ZET)用于发育毒性评估的兴趣呈指数级增长。这一点也从最近关于更新ICHS5指南的提案中可见一斑。不同群体使用的ZET方法差异很大。为了进一步评估其成功程度并使ZET更上一层楼，程序的协调至关重要。在本研究中，基于文献和经验数据，提出了关于温度、测试室、暴露时间、绒毛膜的存在、溶剂的使用、暴露方法、浓度的选择和致畸分类的最优研究设计。此外，我们的形态评分系统作为协议被详细报告，以进一步加强研究设计的一致性。(C)2015 Elsevier Inc.保留所有权利。
In the last couple of years, the interest in the zebrafish embryotoxicity test (ZET) for use in developmental toxicity assessment has been growing exponentially. This is also evident from the recent proposal for updating the ICHS5 guideline. The methodology of the ZET used by the different groups varies greatly. To further evaluate its successfulness and to take the ZET to the next level, harmonization of procedures is crucial. In the present study, based on literature and empirical data, the most optimal study design regarding temperature, test chamber, exposure period, presence of chorion, solvent use, exposure method, choice of concentrations, and teratogenic classification is proposed. Furthermore, our morphology scoring system is reported in detail as protocol to further enhance study design harmonization. (C) 2015 Elsevier Inc. All rights reserved.