In vitro fermentation of sugar beet arabinan and arabino-oligosaccharides by the human gut microflora
In vitro fermentation of sugar beet arabinan and arabino-oligosaccharides by the human gut microflora
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DOI：
10.1111/j.1365-2672.2005.02780.x
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发表时间：
2006-02-01
影响因子：
4
通讯作者：
Rastall, RA
中科院分区：
文献类型：
--
作者：
Al-Tamimi, MAHM;Palframan, RJ;Rastall, RA
目的：测定不同聚合度的阿拉伯低聚糖在人体肠道细菌中的发酵谱。材料和方法：用市售果胶酶对甜菜阿拉伯糖进行酶解，通过凝胶过滤层析分离出8个不同相对分子质量的组分。水解物阿拉伯糖、阿拉伯糖和低聚果糖由肠道细菌厌氧发酵。细菌总数，双歧杆菌，类杆菌，乳杆菌和产气荚膜梭菌/组织溶解亚种。GRP。结果：不同分子量组分对双歧杆菌有不同程度的刺激作用，48h后低分子量组分的双歧杆菌数量增加最大。乳酸菌随初始接种量的不同而波动。类杆菌的数量因组分的不同而不同，阿拉伯糖、阿拉伯糖和较高聚合度的低聚糖(聚合度，DP>；8)在24 h时显著增加，只有DP为1~2的碳水化合物混合物在48 h时显著增加(log8.77+/-0.23)。结论：阿拉伯低聚糖是一种潜在的益生菌。本研究的意义和影响：阿拉伯低聚糖是甜菜工业副产品甜菜浆中的一种成分，因此广泛存在。从阿拉伯多糖中产生益生素功能将为可再生资源带来巨大的附加值。
Aims: To determine the fermentation profiles by human gut bacteria of arabino-oligosaccharides of varying degree of polymerization.Materials and Methods: Sugar beet arabinan was hydrolyzed with a commercial pectinase and eight fractions, of varying molecular weight, were isolated by gel-filtration chromatography. Hydrolysis fractions, arabinose, arabinan and fructo-oligosaccharides were fermented anaerobically by gut bacteria. Total bacteria, bifidobacteria, bacteroides, lactobacilli and the Clostridium perfringens/histolyticum sub. grp. were enumerated using fluorescent in situ hybridization.Results: Bifidobacteria were stimulated to different extents depending on molecular weight, i.e. maximum increase in bifidobacteria after 48 h was seen on the lower molecular weight fractions. Lactobacilli fluctuated depending on the initial inoculum levels. Bacteroides numbers varied according to fraction; arabinan, arabinose and higher oligosaccharides (degree of polymerization, dp > 8) resulted in significant increases at 24 h. Only carbohydrate mixtures with dp of 1-2 resulted in significant increases at 48 h (log 8.77 +/- 0.23). Clostridia decreased on all substrates.Conclusions: Arabino-oligosaccharides can be considered as potential prebiotics.Significance and Impact of the Study: Arabinan is widely available as it is a component of sugar beet pulp, a co-product from the sugar beet industry. Generation of prebiotic functionality from arabinan would represent significant added value to a renewable resource.