Chromatographic separation of germanium and arsenic for the production of high purity (77)As.
Chromatographic separation of germanium and arsenic for the production of high purity (77)As.
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色谱分离锗和砷以生产高纯度 (77)As。
DOI：
10.1016/j.chroma.2016.02.074
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Jurisson,SilviaS
中科院分区：
文献类型：
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作者：
Gott,MatthewD;DeGraffenreid,AnthonyJ;Feng,Yutian;Phipps,MichaelD;Wycoff,DonaldE;Embree,MaryF;Cutler,CathyS;Ketring,AlanR;Jurisson,SilviaS
建立了一种简单的柱层析方法，从锗中分离出77As(94%±16.6%(Etoh/HCl)；74%±11.1%(MeOH))，用于放射免疫治疗。砷和锗的分离是基于不同的色谱材料在水和有机环境中的相对亲和力。使用有机或混合流动相，锗以锗酸盐的形式选择性地保留在硅胶柱上，而砷以砷酸盐的形式从硅胶柱上洗脱出来。随后，用中性到碱性的水溶液洗脱，回收富含的76Ge(98℃±0.2)供重复使用。从甲醇分离的[77As]砷酸盐[17]中观察到放射性标记产率大于98%的a-77As-三硫醇。
A simple column chromatographic method was developed to isolate77As (94 ± 6% (EtOH/HCl); 74 ± 11 (MeOH)) from germanium for potential use in radioimmunotherapy. The separation of arsenic from germanium was based on their relative affinities for different chromatographic materials in aqueous and organic environments. Using an organic or mixed mobile phase, germanium was selectively retained on a silica gel column as germanate, while arsenic was eluted from the column as arsenate. Subsequently, enriched76Ge (98 ± 2) was recovered for reuse by elution with aqueous solution (neutral to basic). Greater than 98% radiolabeling yield of a77As-trithiol was observed from methanol separated [77As]arsenate [17].