THE CHROMOSOMAL CONSTITUTION OF WINE STRAINS OF SACCHAROMYCES-CEREVISIAE
THE CHROMOSOMAL CONSTITUTION OF WINE STRAINS OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1002/yea.320060503
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发表时间：
1990-09-01
期刊：
影响因子：
2.6
通讯作者：
SNOW, R
中科院分区：
文献类型：
--
作者：
BAKALINSKY, AT;SNOW, R
描述了基于对工业实验室菌株杂交体的随机孢子后代中输入标记的分离频率的分析来确定酿酒酵母工业菌株的染色体构成的一般程序。使用的多重营养缺陷型单倍体测试仪携带显性红霉素抗性标记，允许在与野生型工业菌株产生的孢子的大规模交配中选择杂种。对单倍体测试者和每个葡萄酒菌株的未选择的孢子群体之间的许多独立杂交进行分析，区分亲本中的二体、三体和四体染色体补体。讨论了对一类重要的异常分离频率的可能解释。分析结果表明，UCD Enology 522 (Montrachet) 是二倍体，并且可能是 VII 号染色体三体； 522X是二倍体； UCD Enology 505（加州香槟）对于 XVI 染色体是二体性，对于 I、II、VI、VIII、IX、X、XII、XV 染色体是三体性，对于 IV、XI、XIII、XIV 染色体是四体性，对于染色体 V 和 VII 是三体性或四体性； UCD Enology 595（巴斯德香槟）对于染色体 I、II、III、IX、XVI 是二体性，对于染色体 IV、VI、X、XII、XIV、XV 是三体性，对于染色体 V、VIII、XI、XIII 是四体性，对于染色体 VII 是二体性或四体性。
A general procedure is described for determining the chromosomal constitution of industrial strains of Saccharomyces cerevisiae based on analysis of segregation frequencies for input markers among random spore progeny of industrial-laboratory strain hybrids. The multiply auxotrophic haploid testers used carried a dominant erythromycin-resistance marker, allowing hybrids to be selected in mass matings with spores produced by the wild-type industrial strains. Analysis of a number of independent crosses between haploid testers and an unselected population of spores of each wine strain distinguished between disomic, trisomic and tetrasomic chromosomal complements in the parents. Possible explanations for a significant class of aberrant segregation frequencies are discussed. Results of the analysis indicate that UCD Enology 522 (Montrachet) is diploid and possibly trisomic for chromosome VII; 522X is diploid; UCD Enology 505 (California Champagne) is disomic for chromosomes XVI, trisomic for chromosomes I, II, VI, VIII, IX, X, XII, XV, tetrasomic for chromosomes IV, XI, XIII, XIV and either trisomic or tetrasomic for chromosomes V and VII; and that UCD Enology 595 (Pasteur Champagne) is disomic for chromosomes I, II, III, IX, XVI, trisomic for chromosomes IV, VI, X, XII, XIV, XV, tetrasomic for chromosomes V, VIII, XI, XIII and either disomic or tetrasomic for chromosome VII.