Isolation of yeast candidates for efficient sophorolipids production: their production potentials associate to their lineage
Isolation of yeast candidates for efficient sophorolipids production: their production potentials associate to their lineage
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DOI：
10.1080/09168451.2016.1191332
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发表时间：
2016-01-01
影响因子：
1.6
通讯作者：
Ishida, Masashi
中科院分区：
文献类型：
--
作者：
Konishi, Masaaki;Fujita, Manako;Ishida, Masashi
利用液滴塌陷法和薄层层析法从环境样品中分离到11株新的生物表面活性剂产生菌。根据薄层色谱分析，9个优势菌株的糖脂斑点的分离模式对应于由Starmerella bombicola型菌株产生的槐糖脂。两个菌株的斑点图谱保留因子值均小于其它菌株。纯化了两种具有代表性的主要产物，并测定了它们的分子结构。主要产物经鉴定为二乙酰化内酯和酸性槐糖脂。两种化合物的脂肪酸部分估计为17-羟甲基十六碳烯酸。培养4d后，糖脂含量在5.0 ~ 22.9g/L之间。系统发育分析表明，分离菌株分别为Starmerella bombicola和Candida floricola。
Eleven biosurfactant-producing strains were newly isolated from environmental samples using a drop-collapse assay and thin-layer chromatography (TLC). According to the TLC analysis, the separation patterns of the glycolipid spots of nine dominant strains corresponded to that of the sophorolipids produced by a Starmerella bombicola type strain. The retention factor values of the spot patterns of two strains were less than those of the others. Two representative major products were purified, and their molecular structures were determined. The major products were identified as diacetylated lactonic and acidic sophorolipids. The fatty acid moieties of both compounds were estimated to be 17-hydroxymethyl hexadecenoic acid. The amounts of glycolipids ranged from 5.0 to 22.9g/L after 4 d of cultivation. According to a phylogenetic analysis, the strains were identified as Starmerella bombicola and Candida floricola.