[FeFe]-Hydrogenase-Catalyzed H2 production in a photoelectrochemical biofuel cell
[FeFe]-Hydrogenase-Catalyzed H2 production in a photoelectrochemical biofuel cell
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DOI：
10.1021/ja077691k
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发表时间：
2008-02-13
影响因子：
15
通讯作者：
Moore, Thomas A.
中科院分区：
文献类型：
--
作者：
Hambourger, Michael;Gervaldo, Miguel;Moore, Thomas A.
丙酮丁醇梭菌[FeFe]-氢化酶HydA已被研究作为光电化学生物燃料电池中的制氢催化剂。氢化酶被吸附到热解石墨边缘和碳毡电极上。固定化氢酶膜的循环伏安图显示阴极质子还原和阳极氢氧化，与析氢的催化偏置。当对电化学活性表面积进行校正时，两种碳电极的阴极电流密度相似，并且与用铂电极获得的阴极电流密度的40%相似。高表面积碳毡/氢化酶电极随后用作光电化学生物燃料电池中的阴极。在光照下，该装置能够氧化生物燃料基质并将质子还原为氢气。在使用氢化酶或铂阴极的光电化学生物燃料电池中观察到类似的光电流和氢气产生速率。
The Clostridium acetobutylicum [FeFe]-hydrogenase HydA has been investigated as a hydrogen production catalyst in a photoelectrochemical biofuel cell. Hydrogenase was adsorbed to pyrolytic graphite edge and carbon felt electrodes. Cyclic voltammograms of the immobilized hydrogenase films reveal cathodic proton reduction and anodic hydrogen oxidation, with a catalytic bias toward hydrogen evolution. When corrected for the electrochemically active surface area, the cathodic current densities are similar for both carbon electrodes, and similar to 40% of those obtained with a platinum electrode. The high surface area carbon felt/hydrogenase electrode was subsequently used as the cathode in a photoelectrochemical biofuel cell. Under illumination, this device is able to oxidize a biofuel substrate and reduce protons to hydrogen. Similar photocurrents and hydrogen production rates were observed in the photoelectrochemical biofuel cell using either hydrogenase or platinum cathodes.