ER-localized adenine nucleotide transporter ER-ANT1: an integrator of energy and stress signaling in rice
ER-localized adenine nucleotide transporter ER-ANT1: an integrator of energy and stress signaling in rice
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内质网定位的腺嘌呤核苷酸转运蛋白 ER-ANT1：水稻能量和应激信号的整合者
DOI：
10.1007/s11103-016-0540-1
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发表时间：
2016-09
影响因子：
5.1
通讯作者：
Zhang Guiquan
中科院分区：
文献类型：
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作者：
Zhang Xiangqian;Zheng Xu;Ke Shanwen;Zhu Haitao;Liu Fang;Zhang Zemin;Peng Xinxiang;Guo Lin;Zeng Ruizhen;Hou Pei;Liu Ziqiang;Wu Suowei;Song Meifang;Yang Jianping;Zhang Guiquan
大多数环境扰动对光合作用具有直接或间接的有害影响，并且因此对细胞的总体能量状态具有直接或间接的有害影响。尽管我们越来越多地了解收敛能量和压力信号，光合作用，能量和压力信号之间的联系，通过假定的共同节点仍然不清楚。在这里，我们确定了一个内质网（ER）定位腺嘌呤核苷酸转运蛋白1（ER-ANT 1），其缺陷导致幼苗在空气中致死，但在高CO2下可行，表现出典型的光呼吸表型。代谢分析表明，ER-ANT 1的缺失导致蔗糖合成的昼夜节律紊乱，并诱导蔗糖信号通路，表明ER参与了植物生命能量代谢的调节。此外，ER-ANT 1的缺陷引发了内质网应激，激活了植物细胞中未折叠蛋白的反应，表明内质网应激与光呼吸密切相关。这些发现为ER定位的ER-ANT 1在水稻的能量和胁迫信号中的关键作用提供了重要证据。我们的研究结果支持这样的想法，ATP是一个中心信号参与植物对各种压力的反应。
Most environmental perturbations have a direct or indirect deleterious impact on photosynthesis, and, in consequence, the overall energy status of the cell. Despite our increased understanding of convergent energy and stress signals, the connections between photosynthesis, energy and stress signals through putative common nodes are still unclear. Here we identified an endoplasmic reticulum (ER)-localized adenine nucleotide transporter1 (ER-ANT1), whose deficiency causes seedling lethality in air but viable under high CO2, exhibiting the typical photorespiratory phenotype. Metabolic analysis suggested that depletion of ER-ANT1 resulted in circadian rhythm disorders in sucrose synthesis and induced sucrose signaling pathways, indicating that the ER is involved in the regulation of vital energy metabolism in plants. In addition, the defect of ER-ANT1 triggers ER stress and activates the unfolded protein response in plant cells, suggesting ER stress and photorespiration are closely linked. These findings provide an important evidence for a key role of ER-localized ER-ANT1 in convergent energy and stress signals in rice. Our findings support the idea that ATP is a central signal involved in the plant response to a variety of stresses.